Functional effects of diphosphomimetic mutations at cAbl-mediated phosphorylation sites on Rad51 recombinase activity

Brendan Alligand 1, 2 Magali Le Breton 1 Damien Marquis 1 François Vallette 2 Fabrice Fleury 1, *
* Corresponding author
2 CRCNA / Equipe 9 - Apoptose et Progression tumorale
INSERM - Institut National de la Santé et de la Recherche Médicale : U892, IRS-UN - Institut de Recherche en Santé de l'Université de Nantes
Abstract : Homologous Recombination enables faithful repair of the deleterious double strand breaks of DNA. This pathway relies on Rad51 to catalyze homologous DNA strand exchange. Rad51 is known to be phosphorylated in a sequential manner on Y315 and then on Y54, but the effect of such phosphorylation on Rad51 function remains poorly understood. We have developed a phosphomimetic model in order to study all the phosphorylation states. With the purified phosphomimetic proteins we performed in vitro assays to determine the activity of Rad51. Here we demonstrate the inhibitory effect of the double phosphomimetic mutant and suggest that it may be due to a defect in nucleofilament formation.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01814671
Contributor : Elizabeth Bernardo <>
Submitted on : Wednesday, June 13, 2018 - 1:52:39 PM
Last modification on : Monday, September 23, 2019 - 3:32:04 PM
Long-term archiving on : Friday, September 14, 2018 - 2:45:42 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Brendan Alligand, Magali Le Breton, Damien Marquis, François Vallette, Fabrice Fleury. Functional effects of diphosphomimetic mutations at cAbl-mediated phosphorylation sites on Rad51 recombinase activity. Biochimie, Elsevier, 2017, 139, pp.115 - 124. ⟨10.1016/j.biochi.2017.05.020⟩. ⟨inserm-01814671⟩

Share

Metrics

Record views

98