TLR2 and neutrophils potentiate endothelial stress, apoptosis and detachment: implications for superficial erosion

Abstract : AIMS: Superficial erosion of atheromata causes many acute coronary syndromes, but arises from unknown mechanisms. This study tested the hypothesis that Toll-like receptor-2 (TLR2) activation contributes to endothelial apoptosis and denudation and thus contributes to the pathogenesis of superficial erosion. METHODS AND RESULTS: Toll-like receptor-2 and neutrophils localized at sites of superficially eroded human plaques. In vitro, TLR2 ligands (including hyaluronan, a matrix macromolecule abundant in eroded lesions) induced endothelial stress, characterized by reactive oxygen species production, endoplasmic reticulum (ER) stress, and apoptosis. Co-incubation of neutrophils with endothelial cells (ECs) potentiated these effects and induced EC apoptosis and detachment. We then categorized human atherosclerotic plaques (n = 56) based on morphologic features associated with superficial erosion, 'stable' fibrotic, or 'vulnerable' lesions. Morphometric analyses of the human atheromata localized neutrophils and neutrophil extracellular traps (NETs) near clusters of apoptotic ECs in smooth muscle cell (SMC)-rich plaques. The number of luminal apoptotic ECs correlated with neutrophil accumulation, amount of NETs, and TLR2 staining in SMC-rich plaques, but not in 'vulnerable' atheromata. CONCLUSION: These in vitro observations and analyses of human plaques indicate that TLR2 stimulation followed by neutrophil participation may render smooth muscle cell-rich plaques susceptible to superficial erosion and thrombotic complications by inducing ER stress, apoptosis, and favouring detachment of EC.
Type de document :
Article dans une revue
European Heart Journal, Oxford University Press (OUP): Policy B, 2015, 36 (22), pp.1394 - 1404. 〈10.1093/eurheartj/ehv044〉
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01793484
Contributeur : Sandrine Maurice <>
Soumis le : mercredi 16 mai 2018 - 15:55:20
Dernière modification le : jeudi 17 mai 2018 - 01:25:39

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Thibaut Quillard, Haniel Alves Araujo, Gregory Franck, Eugenia Shvartz, Galina Sukhova, et al.. TLR2 and neutrophils potentiate endothelial stress, apoptosis and detachment: implications for superficial erosion. European Heart Journal, Oxford University Press (OUP): Policy B, 2015, 36 (22), pp.1394 - 1404. 〈10.1093/eurheartj/ehv044〉. 〈inserm-01793484〉

Partager

Métriques

Consultations de la notice

39