HLA-E-Restricted Cross-Recognition of Allogeneic Endothelial Cells by CMV-Associated CD8 T Cells: A Potential Risk Factor following Transplantation

Abstract : Although association between CMV infection and allograft rejection is well admitted, the precise mechanisms involved remain uncertain. Here, we report the characterization of an alloreactive HLA-E-restricted CD8 T cell population that was detected in the PBL of a kidney transplant patient after its CMV conversion. This monoclonal CD8 T cell population represents a sizable fraction in the blood (3% of PBL) and is characterized by an effector-memory phenotype and the expression of multiple NK receptors. Interestingly, these unconventional T cells display HLA-E-dependent reactivity against peptides derived from the leader sequences of both various HCMV-UL40 and allogeneic classical HLA-I molecules. Consequently, while HLA-E-restricted CD8 T cells have potential to contribute to the control of CMV infection in vivo, they may also directly mediate graft rejection through recognition of peptides derived from allogeneic HLA-I molecules on graft cells. Therefore, as HLA-E expression in nonlymphoid organs is mainly restricted to endothelial cells, we investigated the reactivity of this HLA-E-restricted T cell population towards allogeneic endothelial cells. We clearly demonstrated that CMV-associated HLA-E-restricted T cells efficiently recognized and killed allogeneic endothelial cells in vitro. Moreover, our data indicate that this alloreactivity is tightly regulated by NK receptors, especially by inhibitory KIR2DL2 that strongly prevents TCR-induced activation through recognition of HLA-C molecules. Hence, a better evaluation of the role of CMV-associated HLA-E-restricted T cells in transplantation and of the impact of HLA-genotype, especially HLA-C, on their alloreactivity may determine whether they indeed represent a risk factor following organ transplantation. Citation: Allard M, Tonnerre P, Nedellec S, Oger R, Morice A, et al. (2012) HLA-E-Restricted Cross-Recognition of Allogeneic Endothelial Cells by CMV-Associated CD8 T Cells: A Potential Risk Factor following Transplantation. PLoS ONE 7(11): e50951.
Type de document :
Article dans une revue
PLoS ONE, Public Library of Science, 2012, 7 (11), pp.50951. 〈10.1371/journal.pone.0050951〉
Liste complète des métadonnées

Littérature citée [52 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01726990
Contributeur : Nathalie Duchange <>
Soumis le : jeudi 8 mars 2018 - 16:55:09
Dernière modification le : vendredi 27 juillet 2018 - 14:36:01
Document(s) archivé(s) le : samedi 9 juin 2018 - 15:50:38

Fichier

journal.pone.0050951.PDF
Publication financée par une institution

Identifiants

Collections

Citation

Mathilde Allard, Pierre Tonnerre, Steven Nedellec, Romain Oger, Alexis Morice, et al.. HLA-E-Restricted Cross-Recognition of Allogeneic Endothelial Cells by CMV-Associated CD8 T Cells: A Potential Risk Factor following Transplantation. PLoS ONE, Public Library of Science, 2012, 7 (11), pp.50951. 〈10.1371/journal.pone.0050951〉. 〈inserm-01726990〉

Partager

Métriques

Consultations de la notice

98

Téléchargements de fichiers

38