Skip to Main content Skip to Navigation
Journal articles

Autologous myoblast transplantation after myocardial infarction increases the inducibility of ventricular arrhythmias

Abstract : BACKGROUND: Small scale clinical trials suggested the feasibility and the efficacy of autologous myoblast transplantation to improve ventricular function after myocardial infarction. However, these trials were hampered by unexpected episodes of life-threatening ventricular tachyarrhythmias (VT). We investigated cardiac electrical stability after myoblast transplantation to the myocardium. METHODS AND RESULTS: Seven days after coronary ligation, Wistar rats were randomized into 3 groups: a control group receiving no further treatment, a vehicle group injected with culture medium into the infarcted myocardium, and a myoblast group injected with autologous myoblasts. Holter monitoring did not discriminate the myoblast from the vehicle groups. Programmed Electrical Stimulation (PES) was performed to evaluate further a cardiac substrate for arrhythmia susceptibility. The occurrence of sustained VT during PES was similar in control and vehicle groups (5/17 and 4/19 rats, respectively; p=0.50). In contrast, 13/20 rats (65%) from the myoblast group showed at least one episode of sustained VT during PES (p<0.05 and p<0.005 versus control and vehicle groups). As a further control group, rats injected with autologous bone marrow mononuclear cells into the infarcted myocardium did not show increased susceptibility to PES. CONCLUSIONS: In an infarcted rat model, myoblast transplantation but not bone marrow mononuclear cells or myocardial injection per se induces electrical ventricular instability. Because ventricular arrhythmias are life-threatening disorders, we suggest that such preclinical evaluation should be conducted for any new source of cells to be injected into the myocardium.
Document type :
Journal articles
Complete list of metadatas

Cited literature [7 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01707095
Contributor : Patricia Lemarchand <>
Submitted on : Monday, February 12, 2018 - 3:09:08 PM
Last modification on : Friday, March 27, 2020 - 2:46:49 AM
Long-term archiving on: : Saturday, May 5, 2018 - 3:58:52 PM

File

Manuscript fernandes CVR-2005-...
Files produced by the author(s)

Identifiers

Collections

Citation

Sarah Fernandes, Jean-Christophe Amirault, Gilles Lande, Jean-Michel Nguyen, Virginie Forest, et al.. Autologous myoblast transplantation after myocardial infarction increases the inducibility of ventricular arrhythmias. Cardiovascular Research, Oxford University Press (OUP), 2006, 69 (2), pp.348 - 358. ⟨10.1016/j.cardiores.2005.10.003⟩. ⟨inserm-01707095⟩

Share

Metrics

Record views

304

Files downloads

722