Temperature-Controlled High-Speed AFM: Real-Time Observation of Ripple Phase Transitions

Abstract : With nanometer lateral and Angstrom vertical resolution, atomic force microscopy (AFM) has contributed unique data improving the understanding of lipid bilayers. Lipid bilayers are found in several different temperature-dependent states, termed phases; the main phases are solid and fluid phases. The transition temperature between solid and fluid phases is lipid composition specific. Under certain conditions some lipid bilayers adopt a so-called ripple phase, a structure where solid and fluid phase domains alternate with constant periodicity. Because of its narrow regime of existence and heterogeneity ripple phase and its transition dynamics remain poorly understood. Here, a temperature control device to high-speed atomic force microscopy (HS-AFM) to observe dynamics of phase transition from ripple phase to fluid phase reversibly in real time is developed and integrated. Based on HS-AFM imaging, the phase transition processes from ripple phase to fluid phase and from ripple phase to metastable ripple phase to fluid phase could be reversibly, phenomenologically, and quantitatively studied. The results here show phase transition hysteresis in fast cooling and heating processes, while both melting and condensation occur at 24.15 °C in quasi-steady state situation. A second metastable ripple phase with larger periodicity is formed at the ripple phase to fluid phase transition when the buffer contains Ca2+ . The presented temperature-controlled HS-AFM is a new unique experimental system to observe dynamics of temperature-sensitive processes at the nanoscopic level.
Type de document :
Article dans une revue
Small, Wiley-VCH Verlag, 2016, 12 (44), pp.6106 - 6113. 〈10.1002/smll.201601549〉
Liste complète des métadonnées

Littérature citée [20 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01653778
Contributeur : Lorena Redondo Morata <>
Soumis le : vendredi 1 décembre 2017 - 19:45:42
Dernière modification le : mardi 30 janvier 2018 - 01:30:35

Fichier

Takahashi_2016_07_27.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Hirohide Takahashi, Atsushi Miyagi, Lorena Redondo-Morata, Simon Scheuring. Temperature-Controlled High-Speed AFM: Real-Time Observation of Ripple Phase Transitions. Small, Wiley-VCH Verlag, 2016, 12 (44), pp.6106 - 6113. 〈10.1002/smll.201601549〉. 〈inserm-01653778〉

Partager

Métriques

Consultations de la notice

118

Téléchargements de fichiers

33