Exposure to Bacterial CpG DNA Protects from Airway Allergic Inflammation by Expanding Regulatory Lung Interstitial Macrophages.

Catherine Sabatel; Coraline Radermecker; Laurence Fievez; Genevieve Paulissen; Svetoslav Chakarov; Claudia Fernandes; Sabine Olivier; Marie Toussaint; Dimitri Pirottin; Xue Xiao; Pascale Quatresooz; Jean-Claude Sirard; Didier Cataldo; Laurent Gillet; Hicham Bouabe; Christophe J Desmet; Florent Ginhoux; Thomas Marichal; Fabrice Bureau

Exposure to Bacterial CpG DNA Protects from Airway Allergic Inflammation by Expanding Regulatory Lung Interstitial Macrophages.

Catherine Sabatel ^{1, 2} Coraline Radermecker ^{2, 1} Laurence Fievez ² Genevieve Paulissen ² Svetoslav Chakarov ³ Claudia Fernandes ² Sabine Olivier ² Marie Toussaint ⁴ Dimitri Pirottin ^{2, 1} Xue Xiao ^{2, 1} Pascale Quatresooz ⁵ Jean-Claude Sirard ⁶ Didier Cataldo ⁷ Laurent Gillet ¹ Hicham Bouabe ⁸ Christophe J Desmet ² Florent Ginhoux ³ Thomas Marichal ^{2, 1} Fabrice Bureau ^{9, 2, 1}

1 University of Liege, Faculty of Veterinary Medecine

2 Cellular and Molecular Immunology, GIGA-Research

3 SIgN - Singapore Immunology Network

4 Imperial College London - National Heart and Lung Institute

5 Service de dermatopathologie

6 CIIL - Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204

7 Laboratory of Tumor and Developmental Biology

8 The Babraham Institute

9 WELBIO - Walloon Excellence in Life sciences and BIOtechnology [Liège]

Abstract : Living in a microbe-rich environment reduces the risk of developing asthma. Exposure of humans or mice to unmethylated CpG DNA (CpG) from bacteria reproduces these protective effects, suggesting a major contribution of CpG to microbe-induced asthma resistance. However, how CpG confers protection remains elusive. We found that exposure to CpG expanded regulatory lung interstitial macrophages (IMs) from monocytes infiltrating the lung or mobilized from the spleen. Trafficking of IM precursors to the lung was independent of CCR2, a chemokine receptor required for monocyte mobilization from the bone marrow. Using a mouse model of allergic airway inflammation, we found that adoptive transfer of IMs isolated from CpG-treated mice recapitulated the protective effects of CpG when administered before allergen sensitization or challenge. IM-mediated protection was dependent on IL-10, given that Il10(-/-) CpG-induced IMs lacked regulatory effects. Thus, the expansion of regulatory lung IMs upon exposure to CpG might underlie the reduced risk of asthma development associated with a microbe-rich environment.

Keywords : CCR2 CpG IL-10 TLR9 asthma hygiene hypothesis lung monocytes regulatory macrophages spleen

Type de document :

Article dans une revue

Immunity, Elsevier, 2017, 46 (3), pp.457-473

Domaine :

Sciences du Vivant [q-bio]

Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-01529380
Contributeur : Jean-Claude Sirard <>
Soumis le : mardi 30 mai 2017 - 16:45:00
Dernière modification le : jeudi 20 septembre 2018 - 13:52:02

Exposure to Bacterial CpG DNA Protects from Airway Allergic Inflammation by Expanding Regulatory Lung Interstitial Macrophages.

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Exposure to Bacterial CpG DNA Protects from Airway Allergic Inflammation by Expanding Regulatory Lung Interstitial Macrophages.

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