Skip to Main content Skip to Navigation
Journal articles

Macrotopographic closure promotes tissue growth and osteogenesis in vitro

Abstract : While the impact of substrate topographies at nano- and microscale on bone cell behavior has been particularly well documented, very few studies have analyzed the role of substrate closure at a tissular level. Moreover, these have focused on matrix deposition rather than on osteoblastic differentiation. In the present work, mouse calvaria cells were grown for 15days on hydroxyapatite (HA) ceramics textured with three different macrogrooves shapes (**100µm): 1 sine and 2 triangle waveforms. We found that macrotopography favors cell attachment, and that bone-like tissue growth and organization are promoted by a tight "closure angle" of the substrate geometry. Interestingly, while Flat HA controls showed little marker expression at the end of the culture, cells grown on macrogrooves, and in particular the most closed (triangle waveform with a 517µm spatial period) showed a fast time-course of osteoblast differentiation, reaching high levels of gene and protein expression of osteocalcin and sclerostin, a marker of osteocytes.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-01484810
Contributor : Laura Juignet <>
Submitted on : Tuesday, March 7, 2017 - 6:00:11 PM
Last modification on : Wednesday, June 24, 2020 - 4:19:31 PM

Identifiers

Citation

Laura Juignet, Baptiste Charbonnier, Virginie Dumas, Wafa Bouleftour, Mireille Thomas, et al.. Macrotopographic closure promotes tissue growth and osteogenesis in vitro. Acta Biomaterialia, Elsevier, 2017, ⟨10.1016/j.actbio.2017.02.037⟩. ⟨inserm-01484810⟩

Share

Metrics

Record views

518