Skip to Main content Skip to Navigation
Journal articles

Simple and fast HPLC method for simultaneous determination of retinol, tocopherols, coenzyme Q10 and carotenoids in complex samples

Abstract : The effects of fat-soluble vitamins (such as vitamins A and E) and lipid microconstituents (such as carotenoids) on human health are now well established. However, high-performance liquid chromatography (HPLC) methods able to detect these molecules in simultaneous runs are often difficult to set up. We report here a 35-min reversed-phase HPLC method using a single C30 column kept at 35 degrees C with a gradient system of methanol, methyl-tert-butyl ether and water at a flow-rate of 1 mL/min. This method resolves 11 carotenoids, retinol, alpha- and gamma-tocopherol from complex matrixes such as food samples, human plasma and human adipose tissue within 35 min. The method is also able to separate coenzyme Q(10). The intra-day and inter-day coefficients of variation are suitable for routine clinical and scientific applications for the determination of lipid micronutrients from various sample types.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-01478665
Contributor : Emmanuelle Reboul <>
Submitted on : Tuesday, February 28, 2017 - 11:48:15 AM
Last modification on : Thursday, July 23, 2020 - 10:14:04 AM

Identifiers

Collections

Citation

Béatrice Gleize, Marlène Steib, Marc André, Emmanuelle Reboul. Simple and fast HPLC method for simultaneous determination of retinol, tocopherols, coenzyme Q10 and carotenoids in complex samples. Food Chemistry, Elsevier, 2012, 134 (4), pp.2560 - 2564. ⟨10.1016/j.foodchem.2012.04.043⟩. ⟨inserm-01478665⟩

Share

Metrics

Record views

180