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Journal articles
Simple and fast HPLC method for simultaneous determination of retinol, tocopherols, coenzyme Q10 and carotenoids in complex samples
Abstract : The effects of fat-soluble vitamins (such as vitamins A and E) and lipid microconstituents (such as carotenoids) on human health are now well established. However, high-performance liquid chromatography (HPLC) methods able to detect these molecules in simultaneous runs are often difficult to set up. We report here a 35-min reversed-phase HPLC method using a single C30 column kept at 35 degrees C with a gradient system of methanol, methyl-tert-butyl ether and water at a flow-rate of 1 mL/min. This method resolves 11 carotenoids, retinol, alpha- and gamma-tocopherol from complex matrixes such as food samples, human plasma and human adipose tissue within 35 min. The method is also able to separate coenzyme Q(10). The intra-day and inter-day coefficients of variation are suitable for routine clinical and scientific applications for the determination of lipid micronutrients from various sample types.
https://www.hal.inserm.fr/inserm-01478665
Contributor : Emmanuelle Reboul Connect in order to contact the contributor
Submitted on : Tuesday, February 28, 2017 - 11:48:15 AM
Last modification on : Thursday, October 21, 2021 - 5:23:47 AM
Contributor : Emmanuelle Reboul Connect in order to contact the contributor
Submitted on : Tuesday, February 28, 2017 - 11:48:15 AM
Last modification on : Thursday, October 21, 2021 - 5:23:47 AM