Skip to Main content Skip to Navigation
Journal articles

Intestinal Scavenger Receptors Are Involved in Vitamin K 1 Absorption

Abstract : Vitamin K-1 (phylloquinone) intestinal absorption is thought to be mediated by a carrier protein that still remains to be identified. Apical transport of vitamin K-1 was examined using Caco-2 TC-7 cell monolayers as a model of human intestinal epithelium and in transfected HEK cells. Phylloquinone uptake was then measured ex vivo using mouse intestinal explants. Finally, vitamin K-1 absorption was compared between wild-type mice and mice overexpressing scavenger receptor class B type I (SR-BI) in the intestine and mice deficient in cluster determinant 36 (CD36). Phylloquinone uptake by Caco-2 cells was saturable and was significantly impaired by co-incubation with alpha-tocopherol (and vice versa). Anti-human SR-BI antibodies and BLT1 (a chemical inhibitor of lipid transport via SR-BI) blocked up to 85% of vitamin K-1 uptake. BLT1 also decreased phylloquinone apical efflux by similar to 80%. Transfection of HEK cells with SR-BI and CD36 significantly enhanced vitamin K-1 uptake, which was subsequently decreased by the addition of BLT1 or sulfo-N-succinimidyl oleate (CD36 inhibitor), respectively. Similar results were obtained in mouse intestinal explants. In vivo, the phylloquinone postprandial response was significantly higher, and the proximal intestine mucosa phylloquinone content 4 h after gavage was increased in mice overexpressing SR-BI compared with controls. Phylloquinone postprandial response was also significantly increased in CD36-deficient mice compared with wild-type mice, but their vitamin K-1 intestinal content remained unchanged. Overall, the present data demonstrate for the first time that intestinal scavenger receptors participate in the absorption of dietary phylloquinone.
Document type :
Journal articles
Complete list of metadatas

Cited literature [40 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01478590
Contributor : Emmanuelle Reboul <>
Submitted on : Wednesday, May 27, 2020 - 9:26:06 AM
Last modification on : Friday, June 26, 2020 - 11:04:03 AM

File

2014_Goncalves_Journal of Biol...
Publisher files allowed on an open archive

Licence


Copyright

Identifiers

Citation

Aurélie Goncalves, Marielle Margier, Stéphanie Roi, Xavier Collet, Isabelle Niot, et al.. Intestinal Scavenger Receptors Are Involved in Vitamin K 1 Absorption. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2014, 289 (44), pp.30743 - 30752. ⟨10.1074/jbc.M114.587659⟩. ⟨inserm-01478590⟩

Share

Metrics

Record views

299

Files downloads

85