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ABCG1 is involved in vitamin E efflux

Abstract : Vitamin E membrane transport has been shown to involve the cholesterol transporters SR-BI, ABCA1 and NPC1L1. Our aim was to investigate the possible participation of another cholesterol transporter in cellular vitamin E efflux: ABCG1. In Abcg1-deficient mice, vitamin E concentration was reduced in plasma lipoproteins whereas most tissues displayed a higher vitamin E content compared to wild-type mice. alpha- and gamma-tocopherol efflux was increased in CHO cells overexpressing human ABCG1 compared to control cells. Conversely, alpha- and gamma-tocopherol efflux was decreased in ABCG1-knockdown human cells (Hep3B hepatocytes and THP-1 macrophages). Interestingly, alpha- and gamma-tocopherol significantly downregulated ABCG1 and ABCA1 expression levels in Hep3B and THP-1, an effect confirmed in vivo in rats given vitamin E for 5 days. This was likely due to reduced LXR activation by oxysterols, as Hep3B cells and rat liver treated with vitamin E displayed a significantly reduced content in oxysterols compared to their respective controls. Overall, the present study reveals for the first time that ABCG1 is involved in cellular vitamin E efflux. (C) 2014 Elsevier B.V. All rights reserved.
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Submitted on : Tuesday, February 28, 2017 - 11:18:38 AM
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Maryline Olivier, Romain Bott, Eric Frisdal, Marion Nowicki, Wanee Plengpanich, et al.. ABCG1 is involved in vitamin E efflux. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, Elsevier, 2014, 1841 (12), pp.1741 - 1751. ⟨10.1016/j.bbalip.2014.10.003⟩. ⟨inserm-01478571⟩



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