Skip to Main content Skip to Navigation
Journal articles

The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability

Abstract : Scope: Vitamin E is present in feed and food mainly as d--tocopherol (d--TOL) but also as all-rac--tocopheryl acetate (rac-alpha-TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion. Methods and results: Using an in vitro digestion model, we showed that TAC was distributed between mixed micelles (36%), liposomes (9%), and nonsolubilized food debris (52%). A significant fraction of TAC was also found in emulsions when fat hydrolysis was not complete. Among the candidate esterases tested, i.e. cholesteryl ester hydrolase, pancreatic lipase, and pancreatic lipase-related protein 2, only cholesteryl ester hydrolase was able to hydrolyze TAC to all-rac-alpha-TOL, about five times more efficiently when it was incorporated into mixed micelles or liposomes than into emulsions or in the food matrix. Caco-2 cells were able to hydrolyze TAC and to uptake TOL when TAC was incorporated into mixed micelles but not into emulsions. Conclusion: During digestion, most TAC is recovered in matrices where its hydrolysis and its uptake by intestinal cells are markedly less efficient than in mixed micelles.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-01478557
Contributor : Patrick Borel <>
Submitted on : Tuesday, February 28, 2017 - 11:13:18 AM
Last modification on : Wednesday, September 16, 2020 - 4:46:33 PM

Links full text

Identifiers

Collections

Citation

Charles Desmarchelier, Franck Tourniaire, Damien P. Prévéraud, Coralie Samson-Kremser, Isabelle Crenon, et al.. The distribution and relative hydrolysis of tocopheryl acetate in the different matrices coexisting in the lumen of the small intestine during digestion could explain its low bioavailability. Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2013, 57 (7), pp.1237 - 1245. ⟨10.1002/mnfr.201200720⟩. ⟨inserm-01478557⟩

Share

Metrics

Record views

174