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. Where, are the the concentrations of the total CIP and metal ions and [CIP] and 524 [I] are the concentrations of the free CIP and metal ions. [CIP:I] is the concentration of the 525 complexe Rearranging eqn (2) with eqn (3) and (4) yields to the quadratic equation (5) from 526 which the eqn (6) is a solution, p.529