Skip to Main content Skip to Navigation
Journal articles

Clinical significance of BRAF mutation status in circulating tumor DNA of metastatic melanoma patients at baseline

Abstract : Circulating tumor DNA is a promising non-invasive tool for cancer monitoring. The main objective of our work was to investigate the relationship between mutant BRAF DNA in plasma and clinical response. Thirty-eight stage IV patients with a V600 mutated BRAF melanoma were included prior to any treatment. DNA was extracted from plasma and mutant DNA was detected using the amplification-refractory mutation system method. Before the beginning of any treatment, the corresponding BRAF mutation was detected in 29 of the 38 tested plasma samples (76.3% positive per cent agreement). We observed a strong correlation between the presence of circulating mutated DNA and overall survival (OS; P=.02), and with the number of metastatic sites (P=.01). The presence of circulating mutated DNA was also strongly correlated with serum LDH activity (P<.01) and S100 protein concentration (P<.01). Finally, seven patients presented discordant BRAF status in different tumor sites. In all these patients, the test performed on ctDNA was positive, suggesting that ctDNA analysis might be less sensitive to tumor heterogeneity. Altogether, these results suggest that plasmatic mutant BRAF DNA is a prognostic factor of OS, correlated with tumor burden. In addition, it represents an interesting alternative source of DNA to detect BRAF mutations before treatment.
Document type :
Journal articles
Complete list of metadatas

Cited literature [22 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01408920
Contributor : Elizabeth Bernardo <>
Submitted on : Monday, December 5, 2016 - 3:03:40 PM
Last modification on : Friday, June 19, 2020 - 3:08:49 AM
Long-term archiving on: : Tuesday, March 21, 2017 - 3:13:16 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Anne Knol, Audrey Vallée, Guillaume Herbreteau, Jean-Michel Nguyen, Emilie Varey, et al.. Clinical significance of BRAF mutation status in circulating tumor DNA of metastatic melanoma patients at baseline. Experimental Dermatology, Wiley, 2016, 25, pp.783 - 788. ⟨10.1111/exd.13065⟩. ⟨inserm-01408920⟩

Share

Metrics

Record views

312