A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

Tung Phan; Brigitte Dreno; Antonio Charlys da Costa; Linlin Li; Patricia Orlandi; Xutao Deng; Beatrix Kapusinszky; Juliana Siqueira; Anne-Chantal Knol; Franck Halary; Jacques Dantal; Kathleen Alexander; Patricia Pesavento; Eric Delwart

doi:10.1016/j.virol.2016.06.013

A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

Tung Phan ^{1, 2} Brigitte Dreno ^{3, 4, *} Antonio Charlys da Costa ^{5, 2} Linlin Li ^{2, 1} Patricia Orlandi ⁶ Xutao Deng ^{2, 1} Beatrix Kapusinszky ^{2, 1} Juliana Siqueira ^{2, 7} Anne-Chantal Knol ^{3, 4} Franck Halary ^{8, 9} Jacques Dantal ¹⁰ Kathleen Alexander ^{11, 12} Patricia Pesavento ¹³ Eric Delwart ^{2, 1, *}

* Auteur correspondant

1 Department of Laboratory Medicine

2 Blood Systems Research Institute

3 CRCNA - Centre de Recherche en Cancérologie / Nantes - Angers

4 Service de dermatologie [Nantes]

5 Institute of Tropical Medicine

6 FIOCRUZ - Fundação Oswaldo Cruz

7 Instituto Nacional de Cancer

8 U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie

9 Service de Transplantation

10 Service de Nephrologie

11 Virginia Tech [Blacksburg]

12 CARACAL

13 Department of Pathology Microbiology and Immunology,

Abstract : We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1–6% nucleotides and shared $ 76% and $ 82% amino acid identity with the NS1 and VP1 of human bufa-viruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epi-dermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cu-tavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined.

Keywords : Oncolytic CTCL Cutavirus Protoparvovirus

Type de document :

Article dans une revue

Virology, Elsevier, 2016, 496, pp.299 - 305. 〈10.1016/j.virol.2016.06.013〉

Domaine :

Sciences du Vivant [q-bio] / Cancer

Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-01408880
Contributeur : Elizabeth Bernardo <>
Soumis le : lundi 5 décembre 2016 - 14:40:31
Dernière modification le : lundi 8 octobre 2018 - 17:44:08
Document(s) archivé(s) le : mardi 21 mars 2017 - 13:09:11

A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

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A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

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