Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Extensive RNA editing and splicing increase immune self-representation diversity in medullary thymic epithelial cells

Abstract : Background: In order to become functionally competent but harmless mediators of the immune system, T cells undergo a strict educational program in the thymus, where they learn to discriminate between self and non-self. This educational program is, to a large extent, mediated by medullary thymic epithelial cells that have a unique capacity to express, and subsequently present, a large fraction of body antigens. While the scope of promiscuously expressed genes by medullary thymic epithelial cells is well-established, relatively little is known about the expression of variants that are generated by co-transcriptional and post-transcriptional processes. Results: Our study reveals that in comparison to other cell types, medullary thymic epithelial cells display significantly higher levels of alternative splicing, as well as A-to-I and C-to-U RNA editing, which thereby further expand the diversity of their self-antigen repertoire. Interestingly, Aire, the key mediator of promiscuous gene expression in these cells, plays a limited role in the regulation of these transcriptional processes.
Document type :
Journal articles
Complete list of metadata

Cited literature [72 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01399345
Contributor : Mireille Bos Connect in order to contact the contributor
Submitted on : Friday, November 18, 2016 - 4:25:36 PM
Last modification on : Tuesday, October 19, 2021 - 11:57:46 PM
Long-term archiving on: : Monday, March 27, 2017 - 9:09:03 AM

File

art_10.1186_s13059-016-1079-9....
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Miri Danan-Gotthold, Clotilde Guyon, Matthieu Giraud, Erez y Levanon, Jakub Abramson. Extensive RNA editing and splicing increase immune self-representation diversity in medullary thymic epithelial cells. Genome Biology, BioMed Central, 2016, 17 (1), pp.219. ⟨10.1186/s13059-016-1079-9⟩. ⟨inserm-01399345⟩

Share

Metrics

Record views

88

Files downloads

138