Broad and Largely Concordant Molecular Changes Characterize Tolerogenic and Immunogenic Dendritic Cell Maturation in Thymus and Periphery

Laurence Ardouin; Hervé Luche; Rabie Chelbi; Sabrina Carpentier; Alaa Shawket; Frédéric Montañana-Sanchis; Camille Santa Maria; Pierre Grenot; Yannick Alexandre; Claude Grégoire; Anissa Fries; Thien-Phong Vu Manh; Samira Tamoutounour; Karine Crozat; Elena Tomasello; Audrey Jorquera; Even Fossum; Bjarne Bogen; Hiroaki Azukizawa; Marc Bajenoff; Sandrine Henri; Marc Dalod; Bernard Malissen

doi:10.1016/j.immuni.2016.07.019

Broad and Largely Concordant Molecular Changes Characterize Tolerogenic and Immunogenic Dendritic Cell Maturation in Thymus and Periphery

Laurence Ardouin ¹ Hervé Luche ^{1, 2} Rabie Chelbi ¹ Sabrina Carpentier ³ Alaa Shawket ¹ Frédéric Montañana-Sanchis ¹ Camille Santa Maria ² Pierre Grenot ² Yannick Alexandre ^{1, 4} Claude Grégoire ¹ Anissa Fries ¹ Thien-Phong Vu Manh ¹ Samira Tamoutounour ¹ Karine Crozat ¹ Elena Tomasello ¹ Audrey Jorquera ¹ Even Fossum ⁵ Bjarne Bogen ⁵ Hiroaki Azukizawa ⁶ Marc Bajenoff ¹ Sandrine Henri ¹ Marc Dalod ^{1, *} Bernard Malissen ^{2, 1, *}

* Auteur correspondant

1 CIML - Centre d'Immunologie de Marseille - Luminy

2 CIPHE - Centre d'Immunophénomique

3 Mi-mAbs (C/O CIML)

CIML - Centre d'Immunologie de Marseille - Luminy

4 VIM - Unité de recherche Virologie et Immunologie Moléculaires

5 Center for Immune Regulation - University of Oslo & Oslo University Hospital Rikshospitalet

6 Department of Dermatology [Osaka, Japan]

Abstract : Dendritic cells (DCs) are instrumental in the initiation of T cell responses, but how thymic and peripheral tolerogenic DCs differ globally from Toll-like receptor (TLR)-induced immunogenic DCs remains unclear. Here, we show that thymic XCR1(+) DCs undergo a high rate of maturation, accompanied by profound gene-expression changes that are essential for central tolerance and also happen in germ-free mice. Those changes largely overlap those occurring during tolerogenic and, more unexpectedly, TLR-induced maturation of peripheral XCR1(+) DCs, arguing against the commonly held view that tolerogenic DCs undergo incomplete maturation. Interferon-stimulated gene (ISG) expression was among the few discriminators of immunogenic and tolerogenic XCR1(+) DCs. Tolerogenic XCR1(+) thymic DCs were, however, unique in expressing ISGs known to restrain virus replication. Therefore, a broad functional convergence characterizes tolerogenic and immunogenic XCR1(+) DC maturation in the thymus and periphery, maximizing antigen presentation and signal delivery to developing and to conventional and regulatory mature T cells.

Type de document :

Article dans une revue

Immunity, Elsevier, 2016, 45 ((2)), pp.305-18. 〈10.1016/j.immuni.2016.07.019〉

Domaine :

Sciences du Vivant [q-bio] / Immunologie

Liste complète des métadonnées

Littérature citée [42 références] Voir Masquer Télécharger

https://www.hal.inserm.fr/inserm-01374574
Contributeur : Sandrine Henri <>
Soumis le : mardi 4 octobre 2016 - 14:00:35
Dernière modification le : mardi 11 décembre 2018 - 13:44:03
Document(s) archivé(s) le : vendredi 3 février 2017 - 15:43:25

Broad and Largely Concordant Molecular Changes Characterize Tolerogenic and Immunogenic Dendritic Cell Maturation in Thymus and Periphery

Fichier

Accès restreint

Identifiants

Collections

Citation

Exporter

Métriques

252

Contact

Broad and Largely Concordant Molecular Changes Characterize Tolerogenic and Immunogenic Dendritic Cell Maturation in Thymus and Periphery

Fichier

Accès restreint

Identifiants

Collections

Citation

Exporter

Partager

Métriques

252

Contact