Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Abstract : AbstractBackgroundAdult skeletal muscles are composed of slow and fast myofiber subtypes which each express selective genes required for their specific contractile and metabolic activity. Six homeoproteins are transcription factors regulating muscle cell fate through activation of myogenic regulatory factors and driving fast-type gene expression during embryogenesis.ResultsWe show here that Six1 protein accumulates more robustly in the nuclei of adult fast-type muscles than in adult slow-type muscles, this specific enrichment takes place during perinatal growth. Deletion of Six1 in soleus impaired fast-type myofiber specialization during perinatal development, resulting in a slow phenotype and a complete lack of Myosin heavy chain 2A (MyHCIIA) expression. Global transcriptomic analysis of wild-type and Six1 mutant myofibers identified the gene networks controlled by Six1 in adult soleus muscle. This analysis showed that Six1 is required for the expression of numerous genes encoding fast-type sarcomeric proteins, glycolytic enzymes and controlling intracellular calcium homeostasis. Parvalbumin, a key player of calcium buffering, in particular, is a direct target of Six1 in the adult myofiber.ConclusionsThis analysis revealed that Six1 controls distinct aspects of adult muscle physiology in vivo, and acts as a main determinant of fast-fiber type acquisition and maintenance.
Type de document :
Article dans une revue
Skeletal Muscle, BioMed Central, 2015, 6 (1), pp.30. 〈10.1186/s13395-016-0102-x〉
Liste complète des métadonnées

Littérature citée [75 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01359830
Contributeur : Bmc Bmc <>
Soumis le : lundi 5 septembre 2016 - 08:04:05
Dernière modification le : vendredi 31 août 2018 - 09:38:13
Document(s) archivé(s) le : mardi 6 décembre 2016 - 12:38:27

Fichiers

13395_2016_Article_102.pdf
Publication financée par une institution

Identifiants

Collections

Citation

Iori Sakakibara, Maud Wurmser, Matthieu Dos Santos, Marc Santolini, Serge Ducommun, et al.. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skeletal Muscle, BioMed Central, 2015, 6 (1), pp.30. 〈10.1186/s13395-016-0102-x〉. 〈inserm-01359830〉

Partager

Métriques

Consultations de la notice

254

Téléchargements de fichiers

104