Comprehensive Proteomic Analysis of Human Erythropoiesis

Abstract : Mass spectrometry-based proteomics now enables the absolute quantification of thousands of proteins in individual cell types. We used this technology to analyze the dynamic proteome changes occurring during human erythropoiesis. We quantified the absolute expression of 6,130 proteins during erythroid differentiation from late burst-forming units-erythroid (BFU-Es) to orthochromatic erythroblasts. A modest correlation between mRNA and protein expression was observed. We identified several proteins with unexpected expression patterns in erythroid cells, highlighting a breakpoint in the erythroid differentiation process at the basophilic stage. We also quantified the distribution of proteins between reticulocytes and pyrenocytes after enucleation. These analyses identified proteins that are actively sorted either with the reticulocyte or the pyrenocyte. Our study provides the absolute quantification of protein expression during a complex cellular differentiation process in humans, and it establishes a framework for future studies of disordered erythropoiesis.
Type de document :
Article dans une revue
Cell Reports , Elsevier Inc, 2016, 16 (5), 〈10.1016/j.celrep.2016.06.085〉
Liste complète des métadonnées

Littérature citée [32 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01356018
Contributeur : Mireille Bos <>
Soumis le : mercredi 24 août 2016 - 15:58:43
Dernière modification le : vendredi 31 août 2018 - 09:13:21
Document(s) archivé(s) le : vendredi 25 novembre 2016 - 13:16:21

Fichier

PIIS2211124716308543.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Emilie-Fleur Gautier, Sarah Ducamp, Marjorie Leduc, Virginie Salnot, François Guillonneau, et al.. Comprehensive Proteomic Analysis of Human Erythropoiesis. Cell Reports , Elsevier Inc, 2016, 16 (5), 〈10.1016/j.celrep.2016.06.085〉. 〈inserm-01356018〉

Partager

Métriques

Consultations de la notice

89

Téléchargements de fichiers

127