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Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts.

Abstract : Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-PfRab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that PfRab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in Apicomplexa parasites.
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Submitted on : Friday, August 19, 2016 - 3:36:37 PM
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David Morse, Wesley Webster, Ming Kalanon, Gordon Langsley, Geoffrey Mcfadden. Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts.. PLoS ONE, Public Library of Science, 2016, 11 (6), pp.e0158174. ⟨10.1371/journal.pone.0158174.s003⟩. ⟨inserm-01354818⟩

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