Immature human dendritic cells enhance their migration through KCa3.1 channel activation

Abstract : Migration capacity is essential for dendritic cells (DCs) to present antigen to T cells for the induction of immune response. The DC migration is supposed to be a calcium-dependent process, while not fully understood. Here, we report a role of the KCa3.1/IK1/SK4 channels in the migration capacity of both immature (iDC) and mature (mDC) human CD14 +-derived DCs. KCa3.1 channels were shown to control the membrane potential of human DC and the Ca 2+ entry, which is directly related to migration capacities. The expression of migration marker such as CCR5 and CCR7 was modified in both types of DCs by TRAM-34 (100 nM). But, only the migration of iDC was decreased by use of both TRAM-34 and KCa3.1 siRNA. Confocal analyses showed a close localization of CCR5 with KCa3.1 in the steady state of iDC. Finally, the implication of KCa3.1 seems to be limited to the migration capacities as T cell activation of DCs appeared unchanged. Altogether, these results demonstrated that KCa3.1 channels have a pro-migratory effect on iDC migration. Our findings suggest that KCa3.1 in human iDC play a major role in their migration and constitute an attractive target for the cell therapy optimization.
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Cell Calcium, Elsevier, 2016, 〈10.1016/j.ceca.2016.02.008〉
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Soumis le : jeudi 19 mai 2016 - 16:12:12
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David Crottès, Romain Félix, Daniel Meley, Stéphanie Chadet, Florence Herr, et al.. Immature human dendritic cells enhance their migration through KCa3.1 channel activation. Cell Calcium, Elsevier, 2016, 〈10.1016/j.ceca.2016.02.008〉. 〈inserm-01318497〉



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