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Glass-LikeMembrane Protein Diffusion in a Crowded Membrane

Abstract : Many functions of the plasma membrane depend critically on its structure and 17 dynamics. Observation of anomalous diffusion in vivo and in vitro using fluorescence 18 microscopy and single particle tracking has advanced our concept of the membrane 19 froma homogeneous fluid bilayer with freely diffusing proteinsto a highly organized 20 crowded and clustered mosaicof lipidsand proteins. Unfortunately, anomalous 21 diffusion could not be related to local molecular details given the lack of direct and 22 unlabeled molecular observation capabilities. Here, we use high-speed atomic force 23 microscopy and a novel analysis methodology to analyze the pore forming protein 24 lysenin in a highly crowded environment and document coexistence of several 25 diffusion regimes within one membrane. We show the formation of local glassy 26 phases, where proteins are trapped in neighbor-formed cages for time scales up to 27 10 seconds, which had not been previously experimentally reported for biological 28 membranes. Furthermore, around solid-like patches and immobile molecules a 29 slower glass phase is detected leading to protein trapping and creating a perimeter 30 of decreased membrane diffusion. 31 32
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Submitted on : Wednesday, March 9, 2016 - 4:50:00 PM
Last modification on : Wednesday, November 3, 2021 - 4:43:14 AM
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Ignacio Munguira, Ignacio Casuso, Hirohide Takahashi, Felix Rico, Mohamed Chami, et al.. Glass-LikeMembrane Protein Diffusion in a Crowded Membrane. ACS Nano, American Chemical Society, 2016, ⟨10.1021/acsnano.5b07595⟩. ⟨inserm-01285787⟩



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