Skip to Main content Skip to Navigation
Journal articles

A lineage-specific methylation pattern controls the transcription of the polycistronic mRNA coding MELOE melanoma antigens

Abstract : We recently characterized two melanoma antigens MELOE-1 and MELOE-2 derived from a polycistronic RNA overexpressed in the melanocytic lineage. This transcription profile was because of hypomethylation of the meloe proximal promoter in melanomas and melanocytes. Here, we investigated whether this demethylation was restricted to the meloe promoter or was linked to a general lack of methylation at the meloe locus in the melanocytic lineage. We established the methylation pattern of the locus spanning more than 40 kbp, focusing on CpG islands, using DNA bisulfite conversion and pyrosequencing. The study was carried out on cultured cell lines (melanoma, melanocyte, colon cancer, and mesothelioma cell lines), healthy tissues (skin and colon), and melanoma tumors. Demethylation, specifically observed in the melanocytic lineage, involves a large promoter area and not the entire meloe locus. This enables updating a tight regulation of meloe transcription in this lineage, suggesting tissue-specific epigenetic mechanisms. Associated with the previously described translational mechanisms, leading to the specific expression of MELOE-1 and MELOE-2 in melanomas, this makes MELOE-derived antigens a relevant candidate for immunotherapy of melanoma. Melanoma Res 00:000–000
Complete list of metadatas

Cited literature [13 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01280880
Contributor : Elizabeth Bernardo <>
Submitted on : Tuesday, March 1, 2016 - 11:54:30 AM
Last modification on : Friday, June 19, 2020 - 3:08:45 AM
Long-term archiving on: : Thursday, November 10, 2016 - 7:08:01 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Benjamin Chalopin, Laetitia Florenceau, Delphine Fradin, Nathalie Labarrière, Agnès Moreau-Aubry. A lineage-specific methylation pattern controls the transcription of the polycistronic mRNA coding MELOE melanoma antigens. Melanoma Research, Lippincott, Williams & Wilkins, 2015, ⟨10.1097/CMR.0000000000000167⟩. ⟨inserm-01280880⟩

Share

Metrics

Record views

249