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Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype

Abstract : AbstractBackgroundFunctional brown adipose tissue (BAT), involved in energy expenditure, has recently been detected in substantial amounts in adults. Formerly overlooked BAT has now become an attractive anti-obesity target.Methods and resultsMolecular characterization of human brown and white adipocytes, using a myriad of techniques including high-throughput RNA sequencing and functional assays, showed that PAZ6 and SW872 cells exhibit classical molecular and phenotypic markers of brown and white adipocytes, respectively. However, the pre-adipocyte cell line SGBS presents a versatile phenotype. A transit expression of classical brown markers such as UCP1 and PPARγ peaked and declined at day 28 post-differentiation initiation. Conversely, white adipocyte markers, including Tcf21, showed reciprocal behavior. Interestingly, leptin levels peaked at day 28 whereas the highest adiponectin mRNA levels were detected at day 14 of differentiation. Phenotypic analysis of the abundance and shape of lipid droplets were consistent with the molecular patterns. Accordingly, the oxidative capacity of SGBS adipocytes peaked on differentiation day 14 and declined progressively towards differentiation day 28.ConclusionsOur studies have unveiled a new phenotype of human adipocytes, providing a tool to identify molecular gene expression patterns and pathways involved in the conversion between white and brown adipocytes.
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Submitted on : Friday, January 29, 2016 - 12:43:30 PM
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Andrea Guennoun, Melissa Kazantzis, Remy Thomas, Martin Wabitsch, Daniel Tews, et al.. Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype. Journal of Translational Medicine, BioMed Central, 2015, 13 (1), pp.135. ⟨10.1186/s12967-015-0480-6⟩. ⟨inserm-01264507⟩



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