Skip to Main content Skip to Navigation
Journal articles

Comprehensive analysis of chemokine-induced cAMP-inhibitory responses using a real-time luminescent biosensor

Abstract : Chemokine receptors are members of the G-protein-coupled receptor (GPCR) family coupled to members of the Gi class, whose primary function is to inhibit the cellular adenylate cyclase. We used a cAMP-related and PKA-based luminescent biosensor (GloSensor™ F-22) to monitor the real-time downstream response of chemokine receptors, especially CX3CR1 and CXCR4, after activation with their cognate ligands CX3CL1 and CXCL12. We found that the amplitudes and kinetic profiles of the chemokine responses were conserved in various cell types and were independent of the nature and concentration of the molecules used for cAMP prestimulation, including either the adenylate cyclase activator forskolin or ligands mediating Gs-mediated responses like prostaglandin E2 or beta-adrenergic agonist. We conclude that the cAMP chemokine response is robustly conserved in various inflammatory conditions. Moreover, the cAMP-related luminescent biosensor appears as a valuable tool to analyze the details of Gi-mediated cAMP-inhibitory cellular responses, even in native conditions and could help to decipher their precise role in cell function.
Document type :
Journal articles
Complete list of metadatas

Cited literature [64 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01229057
Contributor : Philippe Deterre <>
Submitted on : Monday, November 16, 2015 - 12:21:55 PM
Last modification on : Friday, July 17, 2020 - 10:30:03 AM
Long-term archiving on: : Friday, April 28, 2017 - 9:44:53 PM

File

Felouzis_Comprehensive.pdf
Files produced by the author(s)

Identifiers

Citation

Virginia Felouzis, Patricia Hermand, Guy Trambly de Laissardière, Christophe Combadière, Philippe Deterre. Comprehensive analysis of chemokine-induced cAMP-inhibitory responses using a real-time luminescent biosensor. Cellular Signalling, Elsevier, 2015, 28 (1), pp.120-129. ⟨10.1016/j.cellsig.2015.10.011⟩. ⟨inserm-01229057⟩

Share

Metrics

Record views

463

Files downloads

451