Skip to Main content Skip to Navigation
Journal articles

Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice.

Abstract : Experimental studies of Plasmodium parasites that infect humans are restricted by their host specificity. Humanized mice offer a means to overcome this and further provide the opportunity to observe the parasites in vivo. Here we improve on previous protocols to achieve efficient double engraftment of TK-NOG mice by human primary hepatocytes and red blood cells. Thus, we obtain the complete hepatic development of P. falciparum, the transition to the erythrocytic stages, their subsequent multiplication, and the appearance of mature gametocytes over an extended period of observation. Furthermore, using sporozoites derived from two P. ovale-infected patients, we show that human hepatocytes engrafted in TK-NOG mice sustain maturation of the liver stages, and the presence of late-developing schizonts indicate the eventual activation of quiescent parasites. Thus, TK-NOG mice are highly suited for in vivo observations on the Plasmodium species of humans.
Document type :
Journal articles
Complete list of metadata

Cited literature [52 references]  Display  Hide  Download
Contributor : Mireille Bos Connect in order to contact the contributor
Submitted on : Wednesday, September 30, 2015 - 3:17:04 PM
Last modification on : Friday, August 5, 2022 - 3:41:35 AM
Long-term archiving on: : Thursday, December 31, 2015 - 10:35:09 AM


Publisher files allowed on an open archive



Valérie Soulard, Henriette Bosson-Vanga, Audrey Lorthiois, Clémentine Roucher, Jean-François Franetich, et al.. Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice.. Nature Communications, Nature Publishing Group, 2015, 6, pp.7690. ⟨10.1038/ncomms8690⟩. ⟨inserm-01207321⟩



Record views


Files downloads