Positively charged microbubbles to target nucleic acid delivery with ultrasound

Abstract : Nucleic acid delivery in vivo via physical means or non-viral vectors still need improvements in particular to reach deep tissues. Sonoporation is highly advantageous for this purpose as ultrasound can be focalised to a particular tissue leading to targeted gene delivery, without alteration of the environing tissues. Few examples of transfection using cationic microbubbles (MBs) and DNA have been reported. We ought to improve the existing systems by developing positively charged MBs able to oscillate in order to follow their fate in vivo and gain in understanding of the pharmacokinetic of the MBs, then target DNA delivery with ultrasound. After optimisation, we obtained MBs in the 1-3 !m range (98% below 10 !m) able to adsorb nucleic acid on their surface. In vitro parameters have been optimised to obtain in vitro transfection both with DNA and siRNA. The systemic injection in mice led to the observation of these MBs in the liver in less than 10 seconds. This investigation allows to address the key parameters to tentatively obtain reproducible gene transfection in vivo.
Type de document :
Communication dans un congrès
Journées RITS 2015, Mar 2015, Dourdan, France. Actes des Journées RITS 2015, 2015
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http://www.hal.inserm.fr/inserm-01154976
Contributeur : Frédérique Frouin <>
Soumis le : lundi 25 mai 2015 - 20:45:13
Dernière modification le : mercredi 27 juin 2018 - 08:24:02
Document(s) archivé(s) le : jeudi 20 avril 2017 - 08:03:00

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  • HAL Id : inserm-01154976, version 1

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Simona Manta, Anthony Delalande, Gilles Renault, Michel Francis Bureau, Matthias Lebertre, et al.. Positively charged microbubbles to target nucleic acid delivery with ultrasound. Journées RITS 2015, Mar 2015, Dourdan, France. Actes des Journées RITS 2015, 2015. 〈inserm-01154976〉

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