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Type, density, and presentation of grafted adhesion peptides on polysaccharide-based hydrogels control preosteoblast behavior and differentiation.

Abstract : In this work, cell-responsive polysaccharide hydrogels were prepared by a simple procedure based on the sequential bioconjugation and cross-linking of the polysaccharide backbone with bioactive peptides and poly(ethylene glycol)-bis(thiol) (PEG-(SH)2), respectively. Using thiol-ene reactions, we successfully functionalized hyaluronic acid (HA) and carboxymethylcellulose (CMC) with short and long peptides (5-mer and 15-mer derivatives, respectively) derived from adhesive proteins of bone extracellular matrix. The resulting HA-peptide and CMC-peptide conjugates with varying degrees of substitution were then carefully characterized by (1)H NMR spectroscopy to precisely control the peptide density into the hydrogels cross-linked with PEG-(SH)2. Preosteoblast seeded on the hydrogels with controlled identical stiffness spread in a manner that was strongly dependent on ligand density. Surprisingly, increasing the density of the adhesive peptide anchors did not result in a plateau of initial cell spreading but rather in a bell-shaped cell response that varies with the nature of both polysaccharide backbone and functional peptide. Placing the cells under optimal conditions for cell/hydrogel interaction, we showed that in HA hydrogels, the polysaccharide moiety is not solely a passive scaffold that presents the active peptides but is an active player in cell microenvironment to control and sustain cell activity.
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https://www.hal.inserm.fr/inserm-01135586
Contributor : Marc Block <>
Submitted on : Wednesday, March 25, 2015 - 3:09:57 PM
Last modification on : Friday, July 10, 2020 - 5:32:04 PM
Long-term archiving on: : Thursday, July 2, 2015 - 7:33:54 AM

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  • HAL Id : inserm-01135586, version 1
  • PUBMED : 25629300

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Jing Jing, Audrey Fournier, Anna Szarpak-Jankowska, Marc Block, Rachel Auzély-Velty. Type, density, and presentation of grafted adhesion peptides on polysaccharide-based hydrogels control preosteoblast behavior and differentiation.. Biomacromolecules, American Chemical Society, 2015, 16 (3), pp.715-22. ⟨inserm-01135586⟩

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