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CD3(bright) signals on γδ T cells identify IL-17A-producing Vγ6Vδ1(+) T cells.

Abstract : Interleukin-17A (IL-17A) is a pro-inflammatory cytokine that has an important role at mucosal sites in a wide range of immune responses including infection, allergy and auto-immunity. γδ T cells are recognized as IL-17 producers, but based on the level of CD3 expression, we now define the remarkable ability of a CD3(bright) γδ T-cell subset with an effector memory phenotype to rapidly produce IL-17A, but not interferon-γ. CD3(bright) γδ T cells uniformly express the canonical germline encoded Vγ6/Vδ1(+) T-cell receptor. They are widely distributed with a preferential representation in the lungs and skin are negatively impacted in the absence of retinoic acid receptor-related orphan receptor gammat expression or endogenous flora. This population responded rapidly to various stimuli in a mechanism involving IL-23 and NOD-like receptor family, pyrin domain containing 3 (NLRP3)-inflammasome-dependent IL-1β. Finally, we demonstrated that IL-17-producing CD3(bright) γδ T cells responded promptly and strongly to pneumococcal infection and during skin inflammation. Here, we propose a new way to specifically analyze IL-17-producing Vγ6/Vδ1(+) T cells based on the level of CD3 signals. Using this gating strategy, our data reinforce the crucial role of this γδ T-cell subset in respiratory and skin disorders.
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https://www.hal.inserm.fr/inserm-01116326
Contributor : Marie-Hélène Derudas <>
Submitted on : Friday, February 13, 2015 - 10:08:13 AM
Last modification on : Tuesday, November 3, 2020 - 3:30:06 PM
Long-term archiving on: : Sunday, April 16, 2017 - 8:11:31 AM

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Christophe Paget, M T Chow, N A Gherardin, P A Beavis, a P Uldrich, et al.. CD3(bright) signals on γδ T cells identify IL-17A-producing Vγ6Vδ1(+) T cells.. Immunology and Cell Biology, Nature Publishing Group, 2015, 93 (2), pp.198-212. ⟨10.1038/icb.2014.94⟩. ⟨inserm-01116326⟩

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