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Journal articles
RFX6 Regulates Insulin Secretion by Modulating Ca2+ Homeostasis in Human β Cells
Abstract : Development and function of pancreatic β cells involve the regulated activity of specific transcription factors. RFX6 is a transcription factor essential for mouse β cell differentiation that is mutated in monogenic forms of neonatal diabetes. However, the expression and functional roles of RFX6 in human β cells, especially in pathophysiological conditions, are poorly explored. We demonstrate the presence of RFX6 in adult human pancreatic endocrine cells. Using the recently developed human β cell line EndoC-βH2, we show that RFX6 regulates insulin gene transcription, insulin content, and secretion. Knockdown of RFX6 causes downregulation of Ca2+-channel genes resulting in the reduction in L-type Ca2+-channel activity that leads to suppression of depolarization-evoked insulin exocytosis. We also describe a previously unreported homozygous missense RFX6 mutation (p.V506G) that is associated with neonatal diabetes, which lacks the capacity to activate the insulin promoter and to increase Ca2+-channel expression. Our data therefore provide insights for understanding certain forms of neonatal diabetes.
Cited literature [45 references]
https://www.hal.inserm.fr/inserm-01103648
Contributor : Mireille Bos <>
Submitted on : Thursday, January 15, 2015 - 10:50:58 AM
Last modification on : Friday, December 11, 2020 - 6:22:01 PM
Long-term archiving on: : Saturday, April 15, 2017 - 5:42:18 PM
Contributor : Mireille Bos <>
Submitted on : Thursday, January 15, 2015 - 10:50:58 AM
Last modification on : Friday, December 11, 2020 - 6:22:01 PM
Long-term archiving on: : Saturday, April 15, 2017 - 5:42:18 PM
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PIIS2211124714009644.pdf
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