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Journal articles
The Central Role of cAMP in Regulating Plasmodium falciparum Merozoite Invasion of Human Erythrocytes
Abstract : All pathogenesis and death associated with Plasmodium falciparum malaria is due to parasite-infected erythrocytes. Invasion of erythrocytes by P. falciparum merozoites requires specific interactions between host receptors and parasite ligands that are localized in apical organelles called micronemes. Here, we identify cAMP as a key regulator that triggers the timely secretion of microneme proteins enabling receptor-engagement and invasion. We demonstrate that exposure of merozoites to a low K + environment, typical of blood plasma, activates a bicarbonate-sensitive cytoplasmic adenylyl cyclase to raise cytosolic cAMP levels and activate protein kinase A, which regulates microneme secretion. We also show that cAMP regulates merozoite cytosolic Ca 2+ levels via induction of an Epac pathway and demonstrate that increases in both cAMP and Ca 2+ are essential to trigger microneme secretion. Our identification of the different elements in cAMP-dependent signaling pathways that regulate microneme secretion during invasion provides novel targets to inhibit blood stage parasite growth and prevent malaria.
Cited literature [52 references]
https://www.hal.inserm.fr/inserm-01103410
Contributor : Mireille Bos Connect in order to contact the contributor
Submitted on : Wednesday, January 14, 2015 - 4:30:45 PM
Last modification on : Tuesday, October 19, 2021 - 11:57:42 PM
Long-term archiving on: : Wednesday, April 15, 2015 - 11:31:14 AM
Contributor : Mireille Bos Connect in order to contact the contributor
Submitted on : Wednesday, January 14, 2015 - 4:30:45 PM
Last modification on : Tuesday, October 19, 2021 - 11:57:42 PM
Long-term archiving on: : Wednesday, April 15, 2015 - 11:31:14 AM
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journal.ppat.1004520.pdf
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