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Selection of Intracellular Single-Domain Antibodies Targeting the HIV-1 Vpr Protein by Cytoplasmic Yeast Two-Hybrid System

Abstract : The targeting of HIV-1 using antibodies is of high interest as molecular tools to better understand the biology of the virus or as a first step toward the design of new inhibitors targeting critical viral intracellular proteins. Small and highly stable llama-derived single-domain antibodies can often be functionally expressed as intracellular antibodies in the cytoplasm of eukaryotic cells. Using a selection method based on the Sos Recruitment System, a cytoplasmic yeast two-hybrid approach, we have isolated single-domain antibodies able to bind HIV-1 Vpr and Capside proteins in the yeast cytoplasm. One anti-Vpr single domain antibody was able to bind the HIV-1 regulatory Vpr protein in the cytoplasm of eukaryotic cells, leading to its delocalization from the nucleus to the cytoplasm. To our knowledge, this is the first description of a functional single-domain intrabody targeting HIV-1 Vpr, isolated using an in vivo cytoplasmic selection method that alleviates some limitations of the conventional yeast two-hybrid system.
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https://www.hal.inserm.fr/inserm-01093997
Contributor : Mireille Bos <>
Submitted on : Thursday, December 11, 2014 - 2:45:25 PM
Last modification on : Thursday, November 26, 2020 - 9:56:03 AM
Long-term archiving on: : Thursday, March 12, 2015 - 10:50:53 AM

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Julie Matz, Cécile Hérate, Jérôme Bouchet, Nelson Dusetti, Odile Gayet, et al.. Selection of Intracellular Single-Domain Antibodies Targeting the HIV-1 Vpr Protein by Cytoplasmic Yeast Two-Hybrid System. PLoS ONE, Public Library of Science, 2014, 9 (12), pp.e113729. ⟨10.1371/journal.pone.0113729⟩. ⟨inserm-01093997⟩

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