Constitutive AKT activation in follicular lymphoma. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue BMC Cancer Année : 2013

Constitutive AKT activation in follicular lymphoma.

Résumé

Background:The phosphoinositide 3- kinase (PI3K) pathway is involved in the growth of various human cancers,including lymphoid malignancies. However its role in the pathogenesis of follicular lymphoma (FL) has not beenyet described.Methods:To clarify this point, biopsy tissue samples from 38 human FL cases were investigated for PIK3CA somaticmutations in exon 9 and 20 using direct sequencing. The same samples were analyzed using western blotting andimmunohistochemistry to detect expression of AKT, phosphorylated AKT (pAKT), and PTEN proteins. Two cases ofbenign lymphadenitis were used as controls.Results:AKT expression was present in all FL and lymphadenitis cases. 14/38 (37%) FL and 2/2 lymphadenitis casesexpressed pAKT. 9/38 (24%) FL samples showed high level of pAKT, whereas 5/38 (13%) FL cases and 2/2 benignlymphadenitis samples expressed low level of pAKT. PTEN expression was observed in 30/38 (79%) FL and 2/2benign lymphadenitis cases, whereas 8/38 (21%) FL cases showed loss of PTEN expression. 3 cases with positivepAKT did not express PTEN. PIK3CA mutations were not detected in any sample.Conclusions:These data suggest that the PI3K/AKT signaling pathway could be activated in a subset of FL cases,due to either AKT phosphorylation or PTEN downregulation, in the absence of PIK3CA mutations.

Domaines

Cancer
Fichier principal
Vignette du fichier
1471-2407-14-565.pdf (1.47 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

inserm-01093518 , version 1 (10-12-2014)

Identifiants

  • HAL Id : inserm-01093518 , version 1
  • PUBMED : 25096023

Citer

Ouardia I Yahiaoui, Jacques A Nunès, Céline Castanier, Raynier Devillier, Florence Broussais, et al.. Constitutive AKT activation in follicular lymphoma.. BMC Cancer, 2013, pp.565. ⟨inserm-01093518⟩
263 Consultations
153 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More