Spire-1 contributes to the invadosome and its associated invasive properties. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2014

Spire-1 contributes to the invadosome and its associated invasive properties.

Vanessa Lagal
  • Fonction : Auteur
Marie Abrivard
  • Fonction : Auteur
Audrey Perazzi
  • Fonction : Auteur
Sonam Popli
  • Fonction : Auteur
Elodie Verzeroli
  • Fonction : Auteur
Isabelle Tardieux

Résumé

Cancer cells have an increased ability to squeeze through extracellular matrix gaps that they create by promoting proteolysis of its components. Major sites of degradation are specialized micro-domains in the plasma membrane collectively named invadosomes where the Arp2/3 complex and formin proteins cooperate to spatio-temporally control actin nucleation and the folding of a dynamic F-actin core. At invadosomes, proper coupling of exo-endocytosis allows polarized delivery of proteases that facilitate degradation of ECM and disruption of the cellular barrier. We investigated the contribution of the actin nucleator Spire-1 to invadosome structure and function, using Src-activated cells and cancer cells. We found that Spire-1 is specifically recruited at invadosomes and is part of a multi-molecular complex containing Src kinase, the formin mDia1 and actin. Spire-1 interacts with the Rab3A GTPase, a key player in the regulation of exocytosis that is present at invadosomes. Finally, over- and under-expression of Spire-1 resulted in cells with an increased or decreased potential for matrix degradation, respectively, therefore suggesting a functional interplay of Spire-1 with both actin nucleation and vesicular trafficking that might impact on cell invasive and metastatic behavior.
Fichier principal
Vignette du fichier
J Cell Sci-2014-Lagal-328-40.pdf (7.7 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

inserm-01075122 , version 1 (16-10-2014)

Identifiants

Citer

Vanessa Lagal, Marie Abrivard, Virginie Gonzalez, Audrey Perazzi, Sonam Popli, et al.. Spire-1 contributes to the invadosome and its associated invasive properties.. Journal of Cell Science, 2014, pp.328-340. ⟨10.1242/jcs.130161⟩. ⟨inserm-01075122⟩
80 Consultations
209 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More