Evaluation of polyelectrolyte complex-based scaffolds for mesenchymal stem cell therapy in cardiac ischemia treatment.

Caroline Ceccaldi 1, 2, * Raya Bushkalova 1, 2 Chiara Alfarano 2 Olivier Lairez 2 Denis Calise 2 Philippe Bourin 3 Celine Frugier 2 Charlotte Rouzaud-Laborde 2, 4 Daniel Cussac 2 Angelo Parini 2, 4 Brigitte Sallerin 2, 4 Sophie Girod Fullana 1
* Auteur correspondant
1 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux
2 I2MC - Institut des Maladies Métaboliques et Cardiovasculaires
3 Laboratoire de thérapie cellulaire
4 Service de Pharmacie Hospitalière
Abstract : Three-dimensional (3D) scaffolds hold great potential for stem cell-based therapies. Indeed, recent results have shown that biomimetic scaffolds may enhance cell survival and promote an increase in the concentration of therapeutic cells at the injury site. The aim of this work was to engineer an original polymeric scaffold based on the respective beneficial effects of alginate and chitosan. Formulations were made from various alginate/chitosan ratios to form opposite-charge polyelectrolyte complexes (PECs). After freeze-drying, the resultant matrices presented a highly interconnected porous microstructure and mechanical properties suitable for cell culture. In vitro evaluation demonstrated their compatibility with mesenchymal stell cell (MSC) proliferation and their ability to maintain paracrine activity. Finally, the in vivo performance of seeded 3D PEC scaffolds with a polymeric ratio of 40/60 was evaluated after an acute myocardial infarction provoked in a rat model. Evaluation of cardiac function showed a significant increase in the ejection fraction, improved neovascularization, attenuated fibrosis as well as less left ventricular dilatation as compared to an animal control group. These results provide evidence that 3D PEC scaffolds prepared from alginate and chitosan offer an efficient environment for 3D culturing of MSCs and represent an innovative solution for tissue engineering.
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Article dans une revue
Acta Biomaterialia, Elsevier, 2014, 10 (2), pp.901-11. 〈10.1016/j.actbio.2013.10.027〉
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http://www.hal.inserm.fr/inserm-01053518
Contributeur : Marie Francoise Simon <>
Soumis le : jeudi 31 juillet 2014 - 11:32:58
Dernière modification le : jeudi 13 septembre 2018 - 10:50:02

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UNIV-TLSE3 | INC-CNRS

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Caroline Ceccaldi, Raya Bushkalova, Chiara Alfarano, Olivier Lairez, Denis Calise, et al.. Evaluation of polyelectrolyte complex-based scaffolds for mesenchymal stem cell therapy in cardiac ischemia treatment.. Acta Biomaterialia, Elsevier, 2014, 10 (2), pp.901-11. 〈10.1016/j.actbio.2013.10.027〉. 〈inserm-01053518〉

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