Correlative Scanning-Transmission Electron Microscopy Reveals that a Chimeric Flavivirus Is Released as Individual Particles in Secretory Vesicles. - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2014

Correlative Scanning-Transmission Electron Microscopy Reveals that a Chimeric Flavivirus Is Released as Individual Particles in Secretory Vesicles.

(1) , (2) , (1) , (1) , (1) , (2) , (1, 3)
1
2
3

Abstract

The intracellular morphogenesis of flaviviruses has been well described, but flavivirus release from the host cell remains poorly documented. We took advantage of the optimized production of an attenuated chimeric yellow fever/dengue virus for vaccine purposes to study this phenomenon by microscopic approaches. Scanning electron microscopy (SEM) showed the release of numerous viral particles at the cell surface through a short-lived process. For transmission electron microscopy (TEM) studies of the intracellular ultrastructure of the small number of cells releasing viral particles at a given time, we developed a new correlative microscopy method: CSEMTEM (for correlative scanning electron microscopy - transmission electron microscopy). CSEMTEM analysis suggested that chimeric flavivirus particles were released as individual particles, in small exocytosis vesicles, via a regulated secretory pathway. Our morphological findings provide new insight into interactions between flaviviruses and cells and demonstrate that CSEMTEM is a useful new method, complementary to SEM observations of biological events by intracellular TEM investigations.
Fichier principal
Vignette du fichier
Plos_One_Correlative_CSEMTEM_Flavi_-_copie.pdf (3.85 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

inserm-00987879 , version 1 (06-05-2014)

Identifiers

Cite

Julien Gaillard, Sellin Caroline, Georgeault Sonia, Rustem Uzbekov, Lebos Claude, et al.. Correlative Scanning-Transmission Electron Microscopy Reveals that a Chimeric Flavivirus Is Released as Individual Particles in Secretory Vesicles.. PLoS ONE, 2014, 9 (3), pp.e93573. ⟨10.1371/journal.pone.0093573⟩. ⟨inserm-00987879⟩

Collections

INSERM UNIV-TOURS
144 View
326 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More