Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia. - Archive ouverte HAL Access content directly
Journal Articles Particle and Fibre Toxicology Year : 2014

Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.

(1) , (2, 3) , (4) , (4) , (5) , (6) , (7) , (8) , (9) , (10) , (11)
1
2
3
4
5
6
7
8
9
10
11
Barbara Fayard
  • Function : Author
  • PersonId : 943097
Nathalie Herlin-Boime
  • Function : Author
  • PersonId : 954688

Abstract

BACKGROUND: TiO2 particles are commonly used as dietary supplements and may contain up to 36% of nano-sized particles (TiO2-NPs). Still impact and translocation of NPs through the gut epithelium is poorly documented. RESULTS: We show that, in vivo and ex vivo, agglomerates of TiO2-NPs cross both the regular ileum epithelium and the follicle-associated epithelium (FAE) and alter the paracellular permeability of the ileum and colon epithelia. In vitro, they accumulate in M-cells and mucus-secreting cells, much less in enterocytes. They do not cause overt cytotoxicity or apoptosis. They translocate through a model of FAE only, but induce tight junctions remodeling in the regular ileum epithelium, which is a sign of integrity alteration and suggests paracellular passage of NPs. Finally we prove that TiO2-NPs do not dissolve when sequestered up to 24 h in gut cells. CONCLUSIONS: Taken together these data prove that TiO2-NPs would possibly translocate through both the regular epithelium lining the ileum and through Peyer's patches, would induce epithelium impairment, and would persist in gut cells where they would possibly induce chronic damage.
Fichier principal
Vignette du fichier
1743-8977-11-13.pdf (762.22 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S1.PDF (123.46 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S2.PDF (155.25 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S3.PDF (461.83 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S4.PDF (8.06 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S5.PDF (319.22 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S6.PDF (67.84 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13-S7.PDF (400.92 Ko) Télécharger le fichier
Vignette du fichier
1743-8977-11-13.xml (132.03 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Format : Other
Format : Other
Format : Other
Format : Other
Format : Other
Format : Other
Format : Other
Format : Other

Dates and versions

inserm-00971511 , version 1 (03-04-2014)

Identifiers

Cite

Emilie Brun, Frédérick Barreau, Giulia Veronesi, Barbara Fayard, Stéphanie Sorieul, et al.. Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia.. Particle and Fibre Toxicology, 2014, 11 (1), pp.13. ⟨10.1186/1743-8977-11-13⟩. ⟨inserm-00971511⟩
631 View
1476 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More