Impact of manganese on primary hippocampal neurons from rodents. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Hippocampus Year : 2014

Impact of manganese on primary hippocampal neurons from rodents.


Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn(2+) may be toxic at high levels. In this study, we addressed the impact of Mn(2+) on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn(2+) leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn(2+) in both cell types exposed to Mn(2+) concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn(2+) had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn(2+) below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn(2+) also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn(2+) demonstrated here supports their use as a relevant model to study Mn(2+) -induced neurotoxicity. © 2014 Wiley Periodicals, Inc.
Embargoed file
Embargoed file
Ne sera jamais visible

Dates and versions

inserm-00968936 , version 1 (01-04-2014)



Alexia Daoust, Yasmina Saoudi, Jacques Brocard, Nora Collomb, Cécile Batandier, et al.. Impact of manganese on primary hippocampal neurons from rodents.. Hippocampus, 2014, 24 (5), pp.598-610. ⟨10.1002/hipo.22252⟩. ⟨inserm-00968936⟩


630 View
3 Download



Gmail Facebook Twitter LinkedIn More