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PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery.

Abstract : Poly(ADP-ribose) polymerase 3 (PARP-3) is a novel member of the PARP family of enzymes that synthesize poly(ADP-ribose) on themselves and other acceptor proteins. Very little is known about this PARP, which is closely related to PARP-1 and PARP-2. By sequence analysis, we find that PARP-3 may be expressed in two isoforms which we studied in more detail to gain insight into their possible functions. We find that both PARP-3 isoforms, transiently expressed as GFP or FLAG fusions, are nuclear. Detection of endogenous PARP-3 with a specific antibody also shows a widespread nuclear distribution, appearing in numerous small foci and a small number of larger foci. Through co-localization experiments and immunoprecipitations, the larger nuclear foci were identified as Polycomb group bodies (PcG bodies) and we found that PARP-3 is part of Polycomb group protein complexes. Furthermore, using a proteomics approach, we determined that both PARP-3 isoforms are part of complexes comprising DNA-PKcs, PARP-1, DNA ligase III, DNA ligase IV, Ku70, and Ku80. Our findings suggest that PARP-3 is a nuclear protein involved in transcriptional silencing and in the cellular response to DNA damage.
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Contributor : Claude Prigent Connect in order to contact the contributor
Submitted on : Thursday, March 27, 2014 - 9:08:41 AM
Last modification on : Wednesday, March 30, 2022 - 2:35:43 PM

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M. Rouleau, D. Mcdonald, P. Gagné, M.-E. Ouellet, A. Droit, et al.. PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery.. Journal of Cellular Biochemistry, Wiley, 2007, 100 (2), pp.385-401. ⟨10.1002/jcb.21051⟩. ⟨inserm-00966658⟩



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