PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Cellular Biochemistry Year : 2007

PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery.

Abstract

Poly(ADP-ribose) polymerase 3 (PARP-3) is a novel member of the PARP family of enzymes that synthesize poly(ADP-ribose) on themselves and other acceptor proteins. Very little is known about this PARP, which is closely related to PARP-1 and PARP-2. By sequence analysis, we find that PARP-3 may be expressed in two isoforms which we studied in more detail to gain insight into their possible functions. We find that both PARP-3 isoforms, transiently expressed as GFP or FLAG fusions, are nuclear. Detection of endogenous PARP-3 with a specific antibody also shows a widespread nuclear distribution, appearing in numerous small foci and a small number of larger foci. Through co-localization experiments and immunoprecipitations, the larger nuclear foci were identified as Polycomb group bodies (PcG bodies) and we found that PARP-3 is part of Polycomb group protein complexes. Furthermore, using a proteomics approach, we determined that both PARP-3 isoforms are part of complexes comprising DNA-PKcs, PARP-1, DNA ligase III, DNA ligase IV, Ku70, and Ku80. Our findings suggest that PARP-3 is a nuclear protein involved in transcriptional silencing and in the cellular response to DNA damage.

Dates and versions

inserm-00966658 , version 1 (27-03-2014)

Identifiers

Cite

M. Rouleau, D. Mcdonald, P. Gagné, M.-E. Ouellet, A. Droit, et al.. PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery.. Journal of Cellular Biochemistry, 2007, 100 (2), pp.385-401. ⟨10.1002/jcb.21051⟩. ⟨inserm-00966658⟩
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