The PITSLRE/CDK11p58 protein kinase promotes centrosome maturation and bipolar spindle formation.

Clotilde Petretti 1 Matthew Savoian 2 Emilie Montembault 1 David Glover 2 Claude Prigent 1 Régis Giet 1
1 IGDR - Institut de Génétique et Développement de Rennes
2 Department of Genetics
Abstract : The CDK11 (cyclin-dependent kinase 11) gene has an internal ribosome entry site (IRES), allowing the expression of two protein kinases. The longer 110-kDa isoform is expressed at constant levels during the cell cycle and the shorter 58-kDa isoform is expressed only during G2 and M phases. By means of RNA interference (RNAi), we show that the CDK11 gene is required for mitotic spindle formation. CDK11 RNAi leads to mitotic checkpoint activation. Mitotic cells are arrested with short or monopolar spindles. gamma-Tubulin as well as Plk1 and Aurora A protein kinase levels are greatly reduced at centrosomes, resulting in microtubule nucleation defects. We show that the mitotic CDK11(p58) isoform, but not the CDK11(p110) isoform, associates with mitotic centrosomes and rescues the phenotypes resulting from CDK11 RNAi. This work demonstrates for the first time the role of CDK11(p58) in centrosome maturation and bipolar spindle morphogenesis.
keyword : mitosis polo-like kinase γ-tubulin Aurora A
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Article dans une revue
EMBO Reports, EMBO Press, 2006, 7 (4), pp.418-24. 〈10.1038/sj.embor.7400639〉
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http://www.hal.inserm.fr/inserm-00966639
Contributeur : Claude Prigent <>
Soumis le : jeudi 27 mars 2014 - 08:40:02
Dernière modification le : mercredi 16 mai 2018 - 11:22:34

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IGDR | BIOSIT | UR1-UFR-SVE | UNIV-RENNES

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Clotilde Petretti, Matthew Savoian, Emilie Montembault, David Glover, Claude Prigent, et al.. The PITSLRE/CDK11p58 protein kinase promotes centrosome maturation and bipolar spindle formation.. EMBO Reports, EMBO Press, 2006, 7 (4), pp.418-24. 〈10.1038/sj.embor.7400639〉. 〈inserm-00966639〉

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