ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis.

Jean-Michel Saffin 1 Magali Venoux 1 Claude Prigent 2 Julien Espeut 3 Francis Poulat 1 Dominique Giorgi 1 Ariane Abrieu 3 Sylvie Rouquier 1
1 IGH - Institut de génétique humaine
2 IGDR - Institut de Génétique et Développement de Rennes
3 CRBM - Centre de recherches de biochimie macromoléculaire
Abstract : We have identified a unique human microtubule-associated protein (MAP) named ASAP for ASter-Associated Protein. ASAP localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis and directly binds to purified microtubules by its COOH-terminal domain. Overexpression of ASAP induces profound bundling of cytoplasmic microtubules in interphase cells and aberrant monopolar spindles in mitosis. Depletion of ASAP by RNA interference results in severe mitotic defects: it provokes aberrant mitotic spindle, delays mitotic progression, and leads to defective cytokinesis or cell death. These results suggest a crucial role for ASAP in the organization of the bipolar mitotic spindle, mitosis progression, and cytokinesis and define ASAP as a key factor for proper spindle assembly.
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Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2005, 102 (32), pp.11302-7. 〈10.1073/pnas.0500964102〉
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Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 18:13:41
Dernière modification le : mercredi 16 mai 2018 - 11:22:34

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IGDR | BIOSIT | UR1-UFR-SVE | BS | UNIV-MONTPELLIER | UNIV-RENNES

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Jean-Michel Saffin, Magali Venoux, Claude Prigent, Julien Espeut, Francis Poulat, et al.. ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2005, 102 (32), pp.11302-7. 〈10.1073/pnas.0500964102〉. 〈inserm-00966570〉

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