Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure.

Claude Prigent; David Glover; Régis Giet

doi:10.1016/j.yexcr.2004.04.052

Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure.

Claude Prigent ¹ David Glover ² Régis Giet ¹

Abstract : The Nima-related kinase 2 (Nek2) has been implicated in the regulation of centrosome integrity and separation in several species and is a candidate for cell transformation. We now show that reduction of levels of the Drosophila Nek2 by RNAi in cultured cells leads to both dispersal of centrosomal antigens and formation of ectopic bodies of centrosomal antigens. Overexpression of the active DmNek2 kinase resulted in an increase in the number of mitotic cells with fragmented centrosomes. The DmNek2 protein kinase is associated with punctuate bodies within the centrosome consistent with its presence on centrioles. In addition, it is present at lower levels on the midbody during cytokinesis. Midbody association was enhanced following overexpression, whereupon the DmNek2 protein kinase also localised to the cell cortex becoming concentrated in the region of the cleavage furrow in late telophase. Many of such cells showed abnormalities in the organisation of anillin and actin in the cleavage furrow that was associated with formation of ectopic membrane protrusions between each daughter cell. We discuss potential roles for DmNek2 in maintaining centrosome integrity in mitosis, during cytokinesis, and consequently for the fidelity of chromosome segregation.

keyword : Kinase Centrosome Mitosis Cytokinesis

Type de document :

Article dans une revue

Experimental Cell Research, Elsevier, 2005, 303 (1), pp.1-13. 〈10.1016/j.yexcr.2004.04.052〉

Domaine :

Sciences du Vivant [q-bio] / Biologie cellulaire

Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00966561
Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 18:01:21
Dernière modification le : mercredi 16 mai 2018 - 11:23:33

Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure.

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Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure.

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