The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-A.

Anna Castro 1 Suzanne Vigneron 1 Cyril Bernis 1 Jean-Claude Labbé 1 Claude Prigent 2 Thierry Lorca 1
1 Centre de Recherche de Biochimie Macromoléculaire
2 Génétique et Développement
Abstract : We have demonstrated previously that Xenopus Aurora-A is degraded at late mitosis by the APC/Fizzy-Related in a D-Box-dependent manner. Here we demonstrate that, although Aurora-B possesses the same D-Box as Aurora-A, Aurora-B is not degraded by this ubiquitin ligase. We have constructed a chimera Aurora-A/B with the N-terminus of Aurora-A and the C-terminus of Aurora-B and we have examined its degradation by APC/Fizzy-Related. We demonstrate that the N-terminus of Aurora-A confers degradation capacity on the C-terminus of Aurora-B and that this feature is blocked by mutation of the conserved D-Box sequence. We characterize the minimal degradation signal at the N-terminus of Aurora-A and demonstrate that its deletion blocks the degradation of this protein by APC/Fizzy-Related. Thus, we conclude that two different degradation signals are required for proteolysis of Aurora-A. The first one, which we designated D-Box-activating domain, within the N-terminal domain of Aurora-A confers the functionality to the second, a silent D-Box, present within the C-terminus of the kinase.
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Article dans une revue
EMBO Reports, EMBO Press, 2002, 3 (12), pp.1209-14. 〈10.1093/embo-reports/kvf241〉
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http://www.hal.inserm.fr/inserm-00966251
Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 12:32:25
Dernière modification le : mercredi 16 mai 2018 - 11:23:33

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IGDR | BIOSIT | UR1-UFR-SVE | UNIV-RENNES

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Anna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbé, Claude Prigent, et al.. The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-A.. EMBO Reports, EMBO Press, 2002, 3 (12), pp.1209-14. 〈10.1093/embo-reports/kvf241〉. 〈inserm-00966251〉

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