Skip to Main content Skip to Navigation
Journal articles

The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-A.

Abstract : We have demonstrated previously that Xenopus Aurora-A is degraded at late mitosis by the APC/Fizzy-Related in a D-Box-dependent manner. Here we demonstrate that, although Aurora-B possesses the same D-Box as Aurora-A, Aurora-B is not degraded by this ubiquitin ligase. We have constructed a chimera Aurora-A/B with the N-terminus of Aurora-A and the C-terminus of Aurora-B and we have examined its degradation by APC/Fizzy-Related. We demonstrate that the N-terminus of Aurora-A confers degradation capacity on the C-terminus of Aurora-B and that this feature is blocked by mutation of the conserved D-Box sequence. We characterize the minimal degradation signal at the N-terminus of Aurora-A and demonstrate that its deletion blocks the degradation of this protein by APC/Fizzy-Related. Thus, we conclude that two different degradation signals are required for proteolysis of Aurora-A. The first one, which we designated D-Box-activating domain, within the N-terminal domain of Aurora-A confers the functionality to the second, a silent D-Box, present within the C-terminus of the kinase.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-00966251
Contributor : Claude Prigent Connect in order to contact the contributor
Submitted on : Wednesday, June 23, 2021 - 8:11:04 AM
Last modification on : Thursday, June 24, 2021 - 4:54:15 PM
Long-term archiving on: : Friday, September 24, 2021 - 6:13:18 PM

File

2002TLorca_The D Box activatin...
Publisher files allowed on an open archive

Identifiers

Citation

Anna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbé, Claude Prigent, et al.. The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-A.. EMBO Reports, EMBO Press, 2002, 3 (12), pp.1209-14. ⟨10.1093/embo-reports/kvf241⟩. ⟨inserm-00966251⟩

Share

Metrics

Record views

654

Files downloads

18