pEg2 aurora-A kinase, histone H3 phosphorylation, and chromosome assembly in Xenopus egg extract.

Laetitia Scrittori 1 Fabienne Hans 1 Dimitar Angelov 1 Monique Charra 1 Claude Prigent 2 Stefan Dimitrov 1
1 Biologie moléculaire et cellulaire de la différenciation
2 Département de Biologie et Génétique du Développement
Abstract : In eukaryotes cell division is accompanied by phosphorylation of histone H3 at serine 10. In this work we have studied the kinase activity responsible for this histone H3 modification by using cell-free extracts prepared from Xenopus eggs. We have found that the Xenopus aurora-A kinase pEg2, immunoprecipitated from the extract, is able to phosphorylate specifically histone H3 at serine 10. The enzyme is incorporated into chromatin during in vitro chromosome assembly, and the kinetics of this incorporation parallels that of histone H3 phosphorylation. Recombinant pEg2 phosphorylates efficiently histone H3 at serine 10 in reconstituted nucleosomes and in sperm nuclei decondensed in heated extracts. These data identify pEg2 as a good candidate for mitotic histone H3 kinase. However, immunodepletion of pEg2 does not interfere with the chromosome assembly properties of the extract nor with the pattern of H3 phosphorylation, suggesting the existence of multiple kinases involved in this H3 modification in Xenopus eggs. This hypothesis is supported by in gel activity assay experiments using extracts from Saccharomyces cerevisiae.
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Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (32), pp.30002-10. 〈10.1074/jbc.M102701200〉
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Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 11:51:18
Dernière modification le : mercredi 16 mai 2018 - 11:24:08

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Laetitia Scrittori, Fabienne Hans, Dimitar Angelov, Monique Charra, Claude Prigent, et al.. pEg2 aurora-A kinase, histone H3 phosphorylation, and chromosome assembly in Xenopus egg extract.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2001, 276 (32), pp.30002-10. 〈10.1074/jbc.M102701200〉. 〈inserm-00966192〉

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