The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I. - Archive ouverte HAL Access content directly
Journal Articles Gene Year : 1996

The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I.

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Abstract

A cDNA clone coding for DNA ligase I (LigI) was isolated from a Xenopus laevis oocyte cDNA library. The 3766-bp sequence showed a putative ORF capable of encoding a 1070-amino-acid protein whose overall identity with two mammalian sequences is 63%. This identity, however, rises to 72.5% in the C-terminal portion of the protein that contains the active site. Expression of the cDNA in a prokaryotic system produces a protein that is immunologically identical to LigI and can be adenylated. The 180-kDa size of the recombinant protein is similar to the LigI detected in oocyte. Northern blot analysis of ovary and embryo RNAs revealed the expression of two (4.1 and 6 kb) LigI transcripts.
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inserm-00966032 , version 1 (26-03-2014)

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  • HAL Id : inserm-00966032 , version 1
  • PUBMED : 8682316

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David Lepetit, Pierre Thiebaud, Saïd Aoufouchi, Claude Prigent, Rozène Guesné, et al.. The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I.. Gene, 1996, 172 (2), pp.273-7. ⟨inserm-00966032⟩
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