Cyclin B/p34cdc2 triggers phosphorylation of DNA ligase I during Xenopus laevis oocyte maturation.

Saïd Aoufouchi 1 Claude Prigent 1 Chris Ford 2 Pierre Thiebaud 1 Michel Philippe 1 Nadine Theze 1
1 Laboratoire de Biologie et Génétique du Développement
2 Department of Biology, School of Biological Sciences
Abstract : Phosphorylation of DNA ligase I has been analyzed during Xenopus laevis early development. The enzyme, which is involved in DNA replication and DNA repair events, is accumulated during oogenesis to reach a maximum in the stage VI oocyte, and remains at a constant level during maturation. When maturation of the oocyte is induced (in vivo or in vitro), this leads to a post-translational modification of the protein. In stage VI oocytes, a DNA ligase I of apparent molecular mass 180 kDa is detected immunologically whereas a 190-kDa form is found in unfertilized eggs and persists until the tadpole stage. This modification is due to phosphorylation performed by a protein kinase that is turned on 3-4 h after induction of the maturation. Activation of the kinase requires protein synthesis, and appearance of phosphorylated DNA ligase coincides with activation of histone H1 kinase activity. Induction of DNA ligase I modification and maturation are induced in the absence of protein synthesis following injection of maturation promoting factor into oocytes. Immunoprecipitated oocyte DNA ligase I is phosphorylated and its molecular mass modified by purified cyclin B/p34cdc2 in vitro. DNA ligase I phosphorylation is not induced in oocyte extract where only mitogen-activated-protein kinase is induced. Phosphorylation of DNA ligase I induced by cdc2 kinase occurs at the time new DNA replication and recombination activities appear in eggs.
Type de document :
Article dans une revue
European Journal of Biochemistry, Wiley, 1995, 230 (2), pp.491-7
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http://www.hal.inserm.fr/inserm-00966029
Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 08:37:35
Dernière modification le : mercredi 16 mai 2018 - 11:24:08

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  • HAL Id : inserm-00966029, version 1
  • PUBMED : 7607220

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Saïd Aoufouchi, Claude Prigent, Chris Ford, Pierre Thiebaud, Michel Philippe, et al.. Cyclin B/p34cdc2 triggers phosphorylation of DNA ligase I during Xenopus laevis oocyte maturation.. European Journal of Biochemistry, Wiley, 1995, 230 (2), pp.491-7. 〈inserm-00966029〉

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