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Expression of DNA ligases I and II during oogenesis and early development of Xenopus laevis.

Abstract : We have analyzed the expression of DNA ligase I protein during oogenesis and early development of Xenopus laevis. The protein is already present in stage I oocytes and then accumulates throughout oogenesis to reach a steady state level by stage VI. It remains at this level at least until tadpole stage. In stage VI oocytes DNA ligase I protein is almost exclusively localized in the germinal vesicle. We have partially purified a DNA ligase II activity from stage VI oocytes, unfertilized eggs, and stage 8 embryos. An 80-kDa polypeptide can be specifically adenylated in all three purified extracts. It is not recognized by antibodies directed against DNA ligase I and is active on oligo(dT)-poly(rA) substrate. It could therefore represent DNA ligase II protein. The presence of both DNA ligases I and II in oocytes and embryos is inconsistent with the DNA ligase model that had been previously proposed for amphibia.
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Journal articles
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https://www.hal.inserm.fr/inserm-00966022
Contributor : Claude Prigent <>
Submitted on : Wednesday, March 26, 2014 - 8:15:49 AM
Last modification on : Wednesday, May 16, 2018 - 11:24:08 AM

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  • HAL Id : inserm-00966022, version 1
  • PUBMED : 1628756

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Saïd Aoufouchi, Claude Prigent, Nadine Theze, Michel Philippe, Pierre Thiebaud. Expression of DNA ligases I and II during oogenesis and early development of Xenopus laevis.. Developmental Biology, Elsevier, 1992, 152 (1), pp.199-202. ⟨inserm-00966022⟩

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