Changes in the catalytic properties of DNA ligases during early sea urchin development.

Claude Prigent 1 Dominique Maniey 1 Jacques Lefresne 2 David Epel 3 Jacques Signoret 2 Jean-Claude David 1
1 Laboratoire de Biochimie du Développement
2 UNICAEN - Université de Caen Normandie
3 Hopkins Marine Station
Abstract : Two distinct DNA ligases are expressed during early sea urchin embryogenesis. A light form (50 kDa) is found in unfertilized eggs (oocyte form) and a heavier enzyme (110 kDa) is observed at the two-cell stage (embryonic form). The chronology of the change reveals that the embryonic form is detected 90 min after fertilization. After the two proteins were purified, their catalytic properties were studied using different substrates. The oocyte ligase acts only on deoxypolymers while the embryonic form also ligates heteropolymers. The two enzymes were found to undergo both nick and cohesive-end ligation. With different kinds of restriction sites it was observed that the embryonic enzyme could also ligate blunt-ended DNA. These catalytic properties account for sealing of exogenous DNA and concatenation following DNA injection into eggs. The role of the oocyte form of the enzyme is unclear; one speculation is a role in repair of DNA breaks which might accumulate during long-term sperm and oocyte storage in the gonad.
Type de document :
Article dans une revue
Developmental Biology, Elsevier, 1987, 124 (1), pp.281-6
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http://www.hal.inserm.fr/inserm-00966016
Contributeur : Claude Prigent <>
Soumis le : mercredi 26 mars 2014 - 07:59:58
Dernière modification le : mardi 5 juin 2018 - 10:14:24

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  • HAL Id : inserm-00966016, version 1
  • PUBMED : 3666310

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COMUE-NORMANDIE | UNIV-RENNES

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Claude Prigent, Dominique Maniey, Jacques Lefresne, David Epel, Jacques Signoret, et al.. Changes in the catalytic properties of DNA ligases during early sea urchin development.. Developmental Biology, Elsevier, 1987, 124 (1), pp.281-6. 〈inserm-00966016〉

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