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Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued.

Abstract : Knowledge of Aurora A kinase functions is limited to premetaphase events, particularly centrosome maturation, G2/M transition, and mitotic spindle assembly. The involvement of Aurora A in events after metaphase has only been suggested because appropriate experiments are technically difficult. We report here the design of the first human Aurora A kinase (as-AurA) engineered by chemical genetics techniques. This kinase is fully functional biochemically and in cells, and is rapidly and specifically inhibited by the ATP analogue 1-Naphthyl-PP1 (1-Na-PP1). By treating cells exclusively expressing the as-AurA with 1-Na-PP1, we discovered that Aurora A is required for central spindle assembly in anaphase through phosphorylation of Ser 19 of P150Glued. This paper thus describes a new Aurora A function that takes place after the metaphase-to-anaphase transition and a new powerful tool to search for and study new Aurora A functions.
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Submitted on : Monday, March 24, 2014 - 5:21:22 PM
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David Reboutier, Marie-Bérengère Troadec, Jean-yves Cremet, Lucie Chauvin, Vincent Guen, et al.. Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued.. Journal of Cell Biology, Rockefeller University Press, 2013, 201 (1), pp.65-79. ⟨10.1083/jcb.201210060⟩. ⟨inserm-00965126⟩



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