PD01 - Respiratory allergens in human milk: potential impact on susceptibility to allergic airway disease

2045-7022-4-S1-P1 2045-7022 Poster discussion presentation PD01 - Respiratory allergens in human milk: potential impact on susceptibility to allergic airway disease MacchiaverniPatricia RekimaAkila TurfkruyerMathilde MascarellLaurent AiroucheSabi MoingeonPhilippe Adel-PatientKarine Condino-NetoAntonio Annesi-MaesanoIsabella PrescottLSusan TulicKMeri VerhasseltValérie

Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil

EA 6302 "Tolérance Immunitaire", Université de Nice Sophia-Antipolis, Hôpital de l'Archet, Nice, France

Research and Development, Stallergenes SA, Antony, France

INRA, UR496 Immuno-Allergie Alimentaire, CEA/IBiTeC-S/SPI, CEA de Saclay, F-91191 Gif sur Yvette cedex, France

EPAR UMR-S 707 INSERM, France

EPAR UMR-S 707 UPMC Paris6, Medical School Saint-Antoine, Paris, France

School of Pediatrics and Child Health, University of Western Australia, Perth, Australia

The International Inflammation “in-FLAME” Network, Worldwide Universities Network (WUN

Clinical and Translational Allergy 3rd Pediatric Allergy and Asthma Meeting (PAAM)Publication of this supplement was funded by the European Academy of Allergy and Clinical Immunology (EAACI).Meeting abstracts - A single PDF containing all abstracts in this supplement is available here.3rd Pediatric Allergy and Asthma Meeting (PAAM)Athens, Greece

17-19 October 2013

http://www.eaaci-paam.org/ 2045-7022 2014 4 Suppl 1 P1 http://www.ctajournal.com/content/4/S1/P1 10.1186/2045-7022-4-S1-P1 1432014 2014Macchiaverni et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Background

Impact of exposure to environmental allergens during early life on allergic sensitization and disease development is controversial.

Objective

We investigated whether airborne allergen from Dermatophagoides pteronyssinus (Der p), a major cause of allergic asthma, is found in human breast-milk and examined its impact on allergic outcome in a mouse model.

Methods

Der p 1 was quantified in human colostrum and milk samples from Brasil, Australia and France by ELISA. Basophil degranulation assay was used to confirm immunogenicity of Der p. BALB/c mice were fostered by mothers exposed to Der p during lactation. Progeny allergic response to Der p was measured at 6-weeks.

Results

Der p 1 was present in 58% Brazilian, 70% French, and 78% Australian colostrum. Median [Der p 1] was similar between countries (96 pg/mL). In mature milk, Der p1 was found in 55% of samples, median [Der p 1] was 65·9 pg/mL and was significantly lower than in colostrum (p=0·0001). Der p 1-containing milks were able to induce basophils degranulation. Mice breastfed by Der p-exposed mothers had 5-fold increased levels of Der p specific IgE and IgG1 compared to mice breastfed by naïve mothers. Their allergic airway inflammation was not affected.

Conclusion

Early life exposure to ubiquitous respiratory allergens can take place through breastfeeding. An animal model mimicking the human situation shows early life exposure to Der p through milk primes the immune system. The presence of respiratory allergens in breast-milk may be an important factor in driving the early immune function towards allergic disease.