 |
Journal articles
Combining Ca2+ and membrane potential imaging in single neurons.
Abstract : The ability to monitor Ca(2+) signals and membrane potential simultaneously at multiple locations on the same neuron facilitates further progress in our understanding of neuronal function. In particular, this method allows correlation of electrical and chemical signals from multiple sites, including those inaccessible to microelectrodes. This protocol describes a procedure for loading cells with two indicators, a Ca(2+)-sensitive Fura dye and voltage-sensitive JPW1114, together with the equipment required for detecting and imaging the two signals. Potential problems are discussed as well as the capabilities and limitations of the technique.
Cited literature [6 references]
https://www.hal.inserm.fr/inserm-00951450
Contributor : Marco Canepari Connect in order to contact the contributor
Submitted on : Monday, February 24, 2014 - 5:14:31 PM
Last modification on : Thursday, May 20, 2021 - 4:50:02 PM
Long-term archiving on: : Saturday, May 24, 2014 - 12:20:12 PM
Contributor : Marco Canepari Connect in order to contact the contributor
Submitted on : Monday, February 24, 2014 - 5:14:31 PM
Last modification on : Thursday, May 20, 2021 - 4:50:02 PM
Long-term archiving on: : Saturday, May 24, 2014 - 12:20:12 PM
File
Canepari-2013_Combining_Ca2_Pr...
Files produced by the author(s)