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Journal Articles Cold Spring Harbor protocols Year : 2013

Combining Ca2+ and membrane potential imaging in single neurons.

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Abstract

The ability to monitor Ca(2+) signals and membrane potential simultaneously at multiple locations on the same neuron facilitates further progress in our understanding of neuronal function. In particular, this method allows correlation of electrical and chemical signals from multiple sites, including those inaccessible to microelectrodes. This protocol describes a procedure for loading cells with two indicators, a Ca(2+)-sensitive Fura dye and voltage-sensitive JPW1114, together with the equipment required for detecting and imaging the two signals. Potential problems are discussed as well as the capabilities and limitations of the technique.
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Dates and versions

inserm-00951450 , version 1 (24-02-2014)

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Marco Canepari, Kaspar E. Vogt, Michel de Waard, Dejan Zecevic. Combining Ca2+ and membrane potential imaging in single neurons.. Cold Spring Harbor protocols, 2013, 2013 (12), pp.1161-4. ⟨10.1101/pdb.prot073114⟩. ⟨inserm-00951450⟩

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